金雀异黄素抑制大鼠膀胱肿瘤作用的实验研究

The experimental study about genistein inhibition of rats bladder tumor

目的探讨金雀异黄素对N-甲基亚硝基脲(MNU)诱导的SD大鼠膀胱肿瘤的抑制作用。方法将60只大鼠随机分成3组,用MNU诱导成膀胱肿瘤,实验1组和实验2组分别经腹腔注射金雀异黄素0.5、1.0mg/kg,每两日1次;对照组用同样的方式注射生理盐水。利用统计方法和病理分级来分析金雀异黄素对MNU诱导的SD大鼠膀胱肿瘤的抑制作用。结果实验1组和实验2组与对照组比较,在体质量减少值、肿瘤个数、抑瘤率等方面,差异有统计学意义(P<0.05);实验1组和实验2组的癌变率相比,差异有统计学意义(P<0.05)。结论金雀异黄素对大鼠膀胱肿瘤有抑制作用,剂量越大,对肿瘤癌变抑制作用越强。

Objective Investigate the effects about genistein in inhibition of N methyl nitrosourea （MNU）-induced SD rats bladder tumor. Methods 60 SD rats were divided into 3 groups randomly,and all of the 3 groups were treated with MNU to induce urinary bladder tumor. Experimental groupl and 2, respectively, by intraperitoneal injection of genistein of 0.5,1.0 mg/kg, every 2 days,the same way injection of saline control group. The statistical methods and pathology classification were used to analyze the effect of genistein on MNU-induced SD rat bladder tumor inhibition. Results Compared with control group,in four aspects which were eight loss value, tumour number,inhibition rate of tumor and pathology classification, the difference was statistically significant （P〈0.05）. Experimental group 1 and experimental group 2 compared to rate of cancer, the difference was statistically significant （P〈0.05）. Conclusion Genistein inhibited rats bladder tumor,the greater the dose,the stronger the inhibition of tumor cancer.