温度与稀释液pH对猪精液常温保存效果的影响

Influence of Temperature and pH of Diluent on Preservation Efficiency of Boar Semen in Room Temperature

为提高猪精液常温保存的效果,研究不同温度和稀释液pH对猪精液常温保存效果的影响。结果表明:(1)当精子活力分别为50%和30%时,配方Ⅰ与Ⅱ稀释液保存的精液,在温度为15～20℃条件下的精子存活时间均显著高于21～25℃(P<0.05);在pH为6.5～6.9条件下的精子存活时间均显著高于6.0～6.4(P<0.05);(2)精子顶体染色结果表明,适宜温度与pH条件下,精液保存48h后,顶体完整率达到96%以上。研究结果表明,猪精液常温保存适宜温度为15～20℃,适宜PH为6.5～6.9。

To improve the preserving efficiency of boar semen in room temperature and ensure the fer- tilizing activity during semen dilution, the preserving conditions of boar semen, including temperature and pH of dilution buffer were optimized. The results showed that the survival time of the sperms in 15℃--20℃ was obviously longer than that in 21℃--25℃ （P〈0.05） when the sperms were stored in buffer I or U with a respective motility rate of 50% and 30%. Similarly, the survival time in pH 6.5--6.9 was longer than that in pH 6.0-6.4 （P〈0.05）. It suggested that the preserving conditions of boar semen was in 15℃-20℃ and in pH 6.5--6.9. Sperm acrosome staining results also showed that acrosome intact rate reached more than 96 % after 48h semen preservation in optimum temperature and pH.