单分子力谱研究活细胞上多药耐药相关蛋白1的表达

Investigation of MRP1 Molecules on Cell Membrane Based on Single Molecule Atomic Force Microscopy

采用原子力显微镜的单分子力谱(SMFM)技术研究了多药耐药相关蛋白1(MRP1)与其抗体间的相互作用,并考察了人舌癌细胞系TCA8113经高剂量平阳霉素(BLM)反复间歇诱导前后细胞表面MRP1的表达差异.实验结果表明,MRP1与其抗体之间存在特异性相互作用力,当针尖运动速率为2.5μm/s时,作用力大小约为(182±35)pN;而且药物诱导后MRP1在人舌癌细胞上的表达明显增强.本工作为了解活细胞水平上MRP1的表达提供了新方法,有助于肿瘤细胞多药耐药性(MDR)的研究.

Multidrug resistance（MDR） is one of the main factors in the failure of chemotherapy against tumors,and multidrug resistance-associated protein 1（MRP1） is closely related to the generation of MDR.In this work,the interaction force between MRP1 and anti-MRP1 was measured via single-molecule force microscopy（SMFM）,and the expression differences of MRP1 on human tongue cancer cells before and after treatment with high-dose bleomycin（BLM） were investigated.The results show that the interaction force between MRP1 and anti-MRP1 is about（182±35） pN as the loading rate is 2.5 μm/s.The expression of MRP1 on human tongue cancer cell membrane increased obviously after treatment with high-dose BLM.This work may provide a new method for studying the expression of MRP1 at the living cell level and can be helpful for the study of MDR.