天竺桂挥发油对小鼠的体外抗氧化作用研究

Effects of essential oils from Cinnamomum japonicum Sibe on antioxidant in mice in vitro

目的:通过提取天竺桂挥发油,对小鼠进行体外的抗氧化的实验,研究天竺桂挥发油的抗氧化作用。方法:使用ROS测定试剂盒测定挥发油的抗活性氧能力;使用丙二醛(MDA)测定试剂盒和GSH-Px活力测定试剂盒测定天竺桂挥发油对小鼠体外MDA含量的影响;用分光光度法来测定天竺桂挥发油对H2O2所诱导的小鼠红细胞溶血和Fe2+-Vc诱导的肝线粒体肿胀度的抑制效果;通过化学方法考查了天竺桂挥发油清除DPPH自由基以及羟自由基的能力。结果:挥发油在浓度为0.25%～1.00%的范围内,有较强的体外抗氧化性,且能减少小鼠肝匀浆和心脏匀浆MDA的含量,对H2O2诱导的小鼠红细胞溶血和由Fe2+-Vc诱导的肝线粒体肿胀度均具有显著的抑制作用,具有较强的清除DPPH自由基以及羟自由基的能力。

Purpose： Through essential oils to extract from Cinnamomum japonicum Sibe, the antioxidant experiments in mice in vitro, studies the constituents of volatile oil from essential oils antioxidant effect. Methods： Using malondialdehyde （MDA） test kit and GSH-Px activity assay kit for determination of essential oils the influence in vitro MDA content in mice; Use spectrophotometric method for determination of H2O2 essential oils that mice induced by red hemolysis and Fe^2＋-Vc induced liver mitochondria swelling degree inhibition effect. Through the chemical method examines essential oils DPPH and hydroxyl radicals clearly the ability of free radicals. Results： In the concentration of volatile oil 0.25%-1.00% range, has the strong external oxidation resistance, and can reduce mouse liver homogenate and heart, the content of MDA homogenate on H2O2 mice induced by red hemolysis and Fe^2＋-Vc induced liver mitochondria swelling degree have significant inhibitory effect, strong scavenging ability of hydroxyl radicals and DPPH radical.