高效液相色谱法测定红茶菌中的功能因子

Analysis of functional factors in Kombucha by reverse phase high performance liquid chromatography

建立了一种利用高效液相色谱法同时分析红茶菌中2种主要功能因子(D-葡萄糖二酸1,4内酯和D-葡萄糖二酸)的方法。采用AgilentSB-Aq色谱柱(4.6mm×250mm,5μm),色谱条件:流动相为0.025mol/LKH2PO4(pH2.5),流速0.5mL/min,柱温30℃,进样量10μL,紫外检测波长为210nm。在此检测条件下,红茶菌中的2种主要功能因子能够得到较好分离,回收率为99.7%～102.3%,RSD<3%,精密度较高,重现性较好。

A method for simultaneous determination of 2 functional factors（D-Saccharic acid 1,4 lactone and D-Saccharic acid）in Kombucha by reverse phase high performance liquid chromatography was developed. Agilent SB-Aq column（4.6 mm×250 mm, 5 μm） was used at 30 % and mobile phase was 0.025 mol/L KH2PO4（pH2.5） with flow rate of 0.5 mL/min. Detection wavelength was 210 nm. Sample size was 10 μL. The relative standard deviations were lower than 3%, and the recoveries were 99.7% to 102.3%.