高效液相色谱法测定低聚半乳糖的含量

Determination of galactooligosaccharide by high performance liquid chromatography

研究了高效液相色谱法测定乳制品及糖浆中低聚半乳糖含量的方法。以水和乙腈为流动相,用氨基柱将葡萄糖,半乳糖和乳糖与其他物质分离,用示差折光检测器检测,定量。通过计算低聚半乳糖酶解成的半乳糖含量,来对低聚半乳糖定量。葡萄糖,半乳糖,乳糖和低聚半乳糖的相对标准偏差分别为2.72%、4.16%、1.16%、4.26%。此法的线性相关系数为0.9990～0.9995,回收率达到95%～110%。此法能快速准确的测定低聚半乳糖的含量。

HPLC method was established to determine the galactooligosaccharide in dairy products and syrup. Glucose, galactose and lactose were simultaneously assayed on a NH2 column by using a refractive index detector and a mobile phase of acetonitrile-water. β-Galactosidase can hydrolyze GOS into galactose. Then GOS are calculated from concentrations of lactose and galactose. The relative standard deviation was 2.72%, 4.16%, 1.16%, 4.26% for glucose, galactose, lactose and galactooligosaccharide respectively. The method presented linear correlation coefficient between 0.9990 and 0.9995. The recovery ranged from 95% to 110%. This method was proved to be accurate and rapid.