L-精氨酸对兔库血输注模型微循环状态的影响

The role of L-Arg on microcirculation in rabbits receiving banked blood transfusion

目的观察L-精氨酸(L-Arg)对兔库血输注模型微环境状态的影响。方法大白兔20只用于制备库血,另40只随机均分为四组:输血组(T组)、L-Arg组(L组)、输血+L-Arg组(TL组)以及空白对照组(C组)。分别于放血前后及输血前后行动脉血气分析,并测量血浆一氧化氮(NO)、内皮素(ET)-1含量。结果 T组的NO和ET-1均于放、输血后升高,而NO/ET-1和pH下降(P<0.05)。TL组出现类似的变化,但其相应时点的NO、ET-1及乳酸含量均低于T组,而NO/ET-1和pH高于T组(P<0.05)。结论补充外源性L-Arg可升高NO水平并抑制ET-1的合成和分泌,且对维持内环境稳定有重要意义。

Objective To evaluate the effect of L-Arg on microenvironment in acute hemorrhagic rabbits receiving banked-blood transfusion. Methods Sixty New Zealand rabbits were included in the present study. Twenty were randomly selected for banked blood. The other 40 rabbits were randomly divided into four groups： transfusion group （T group, n = 10）, L-Arg group （L group, n=10）, transfusion/L-Arg group （TL group, n = 10）, control group （C group, n = 10）. Arterial blood gas, plasma NO and ET-1 concentration were measured and analyzed before （To） and after haemospasia （T1）, at the end of transfusion （T2）, 1 h （T3） and 2 h （r4） after transfusion. Results NO and ET-1 levels of T group were significantly elevated after haemospasia and blood transfusion （P〈0.05）, whereas NO/ET-1 and pH value were significantly decreased （P〈0. 05）. The levels of NO, ET-1, NO/ET-1, and pH values in TL group were changed similarly to those in T group （P〈0.05）. However, the levels of NO, ET-1, and lactic acid were significantly lower than those of T group at corresponding time point （P〈0.05）. NO/ET-1 and pH values of TL group were significantly higher than those of T group （P〈0.05）. Conclusion Intravenous L-Arg 300 mg/kg may increase the level of NO and inhibit the production of ET-1, which is beneficial for homeostasis.