发酵生产脂肪酶的菌株筛选及其应用

Screening and Application of a Lipase-Producing Strain

采用从大庆日月星油厂附近的土壤中筛选得到产脂肪酶的菌株xjA,在固态发酵的条件下生产脂肪酶,并将所产脂肪酶应用于合成共轭亚油酸甘油酯。本实验利用单因素和正交试验方法确定产脂肪酶的最佳固态发酵条件:在种龄36h的情况下,碳源(麸皮)与氮源(豆粕)质量比(m麸皮:m豆粕)为1:4、V培养基:V加水量1:1、接种量0.3%、发酵温度29℃、发酵时间3d。在此条件下进行固态发酵,固态培养基中脂肪酶的活力最高,可达1450U/g;所得脂肪酶用于合成共轭亚油酸甘油酯,产物中共轭亚油酸(CLA)接入率可达到13.6%。

In this study, a lipase-producing strain, named as xjA, was isolated from soil near Riyuexing oil factory in Daqing. The solid-state fermentation conditions for lipase production by the strain were optimized using one-factor-at-a-time and orthogonal array design methods. Meanwhile, the prepared lipase was used to synthesize conjugated linoleic acid glyceride. The results indicated that the optimal fermentation conditions were wheat bran/soybean meal mass ratio of 1：4, culture medium/adding water volume ratio of 1：1, fermentation temperature of 29 ℃, inoculation amount of 0.3%, and fermentation time of 3 days. Under these conditions, the activity of lipase was up to 1450 U/g. The load rate of conjugated linoleic acid （CLA） in the conjugated linoleic acid glyceride synthesized using the obtained lipase was 13.6%.