枯草芽孢杆菌JMUKC2产肌苷摇瓶发酵培养基的优化

Culture Medium Optimization for Inosine Production by Bacillus subtilis JMUKC2 in Shake Flasks

利用单因素试验和正交试验对肌苷生产菌枯草芽孢杆菌JMUKC2的摇瓶发酵培养基进行优化,优化后的最佳发酵培养基配方为葡萄糖140g/L、玉米浆20g/L、酵母膏17g/L、尿素7g/L、硫酸铵20g/L、MgSO4.7H2O1g/L、KH2PO47g/L、CaCO320g/L。培养基经优化后,生物量比优化前提高了40.67%,肌苷产量比优化前提高了46.57%。

This paper describes the optimization of the fermentation medium for inosine-overproducing Bacillus subtilis JMUKC2 in shakes flask using one-factor-at-a-time and orthogonal array design methods. The optimal fermentation medium was composed of 140 g/L glucose, 20 g/L corn liquor, 17 g/L yeast, 7 g/L urea, 20 g/L ammonium sulfate, 1 g/L MgSO4 o7H20, 7 g/L KH2PO4 and 20 g/L CaCO3. The resulting biomass and inosine production were increased by 40.67% and 46.57% compared with those before the optimization, respectively.