摘要
为提高虎杖中白藜芦醇得率,采用虎杖内生真菌直接发酵虎杖转化白藜芦醇苷为白藜芦醇,通过从虎杖根、茎中初筛得到6株内生真菌,复筛以虎杖煮提液为底物发酵,采用高效液相色谱法定量检测发酵液中白藜芦醇苷和白藜芦醇含量。结果表明:6株内生真菌均具有转化白藜芦醇苷为白藜芦醇能力,其中分离于茎内的J1转化率最高为25.6%。J1经形态学观察及ITS-5.8S rDNA序列分析,鉴定为草酸青霉Penicillium oxalicum,Genbank登录号为HQ732137。
In order to improve the yield of resveratrol from Polygonum cuspidatum through endophytic fungi transformation, endophytic fungal strains capable of transforming polydatin from Polygonum cuspidatum were screened. The contents of resveratrol and polydatin were determined by high performance liquid chromatography （HPLC）. The results indicated that 6 endophytic fungal strains capable of transforming polydatin from Polygonum cuspidatum were identified. Strain J1 had the highest transformation rate, 25.6%. The strain J1 was identified as Penicillium oxalicum with Genbank accession number of HQ732137 through morphologic observation and ITS-5.8S rDNA sequence analysis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第11期172-176,共5页
Food Science
基金
湖南省博士后科研资助专项计划项目(2010RS4003)
长沙市科技局一般项目(K1005013-31)
湖南省中医药科研计划项目(2010079)
关键词
虎杖
白藜芦醇苷
白藜芦醇
内生真菌
分离
鉴定
Polygonum cuspidatum
polydatin
resveratrol
endophytic fungi
isolation： identification
