微纳米生物玻璃的体外成骨性能研究

Biocompatibility and Osteogenesis of Micro-and Nano-Bioactive Glasses

微纳米生物活性玻璃因其具有特殊的形态结构和理化性能目前引起众多研究者的关注,但是目前对微纳米生物活性玻璃的结构及形态对细胞性能的影响研究的较少。本文通过溶胶-凝胶法结合模板仿生技术合成了具有特殊结构和形态的微纳米生物活性玻璃,并通过体外细胞实验,研究了这种微纳米结构对人骨髓间充质干细胞成骨性能(ALP,Runx2,Col1a1和OPN)的影响,结果证明具有规则形态的生物活性玻璃(SBG)相比不规则形态的生物活性玻璃(IBG)更能促进细胞的体外成骨性能,为将来设计具有特定结构的生物活性玻璃提供了参考依据。

Because of the special morphology and characterization, micro- and nano- bioactive glass caused much atten- tion by researchers, but the morphology of micro- and nano-bioactive glass on cell performance has been underappreciated and underinvestigated. The special morphology of micro- and nano-bioactive glass was synthesized by the Sol-gel method combined with the template bionic. Human mesenchymal stem cells （hMSCs） were seeded on bioactive glass for 6 days and 10 days in vitro for alkalic phosphatase （ALP） activity and 5days for osteogenic gene expression analysis. Based on the results of ALP activity and osteogenic gene expression analysis, the responses of hMSCs to the SBG exhibited a higher degree of osteogenic differentiation than those on IBG in vitro. This work will provide a reference for design of specific mor- phology of the bioactive glass in the future.