小分子硼酸肽的自组装

Self-Assembly of Oligo-Borono-Peptides

利用FMOC化学固相多肽合成法合成了3种含精氨酸的小分子硼酸肽(标记为BPs(1-3))。在生理pH下,含阳离子的硼酸肽可自组装形成有序超分子纳米组装体。二羟基酚染料茜素红与硼酸肽可特异性结合形成五元环硼酸酯,伴随荧光和颜色的显著变化,可进一步调控硼酸肽的自组装行为。通过扫描电镜研究茜素红调控前后硼酸肽的自组装形态,并用红外光谱和圆二色谱研究其自组装机理。结果表明,3种含精氨酸硼酸肽在生理pH下可自组装形成不同的超分子纳米组装体。通过茜素红的调控,茜素红/硼酸肽化合物,可自组装形成更有序,更精致的超分子聚集体。

Three arginine-containing oligo-borono-peptides （BPs （1-3）） were synthesized by employing a standard FMOC chemistry SPPS. BPs（1-3） could spontaneously self-assemble into supramolecular architectures at a physiological environment. With recognition between BPs and alizarin red S （ARS） , the five-membered ring borate esters （ARS/BPs） could be formed with dramatic changes in fluorescent feature and visible color. These self-assembled supramolecular archi- tectures were observed by scanning electron microscope （SEM） and their self-assembly mechanisms were explored by Fou- rier Transform Infrared Spectroscopy （FT-IR） and Circular Dichroism （CD）. It was found that BPs were capable of self- assembling into well-ordered supramolecular architectures, and after recognition of ARS, ARS/BPs could further self-as- semble into more ordered and sophisticated supramolecular architectures.