微粒体环氧化酶与谷胱甘肽S转移酶的遗传多态性对新生儿出生体重的影响

Association Between Gegentic Polymorphisms of Microsomal Epoxide Hydrolase and the Glutathione S transferases in Mother and Their Newborn Birth Weights

目的 ]探讨母亲微粒体环氧化酶 (EPHX1)与谷胱甘肽 S转移酶 (GSTM1,GSTT1)的遗传多态性对新生儿出生体重的影响。 [方法 ]采用回顾性流行病学调查方法 ,使用统一调查表 ,由经过培训的调查员在北京燕山某医院对入院分娩的 6 30个母亲婴儿对进行调查。 [结果 ]单因素分析发现 :EPHX1和 GSTT1、GSTM1基因多态对新生儿出生体重的影响无显著意义。经母亲年龄、文化程度、生育史、芳香烃溶剂暴露、在家被动吸烟、孕前体重指数、新生儿孕周与性别因素调整后 ,也未见有显著意义。然而对基因 EPHX1,GSTT1,和 GSTM1综合分析时发现 :EPHX1杂合子 /突变基因型合并 GSTT1缺失基因型的母亲其新生儿出生体重显著低于 EPHX1纯合子 /野生型合并GSTT1存在基因型 ;EPHX1杂合子 /突变基因型合并 GSTM1缺失基因型的母亲其新生儿体重显著低于 EPHX1纯合子 /野生基因型合并 GSTM1存在基因型。 [结论 ]基因 EPHX1与基因 GSTT1,GSTM1之间对新生儿出生体重的影响存在交互作用 。

Objective]To investigate association between genetic polymorphisms of microsomal epoxide hydrolase(EPHX1) and the glutathione S-transferases (GSTM1 and GSTT1) in mother and newborn birth weights [Methods]Using standard questionaire,630 singleton live born infant-mother pairs were investigated by trained field workers with retrospective epidemiological method in a certain hospital in Yanshan of Beijing,China.[Results]Both EPHX1 and GSTT1, GSTM1 polymorphisms did not significantly associated with reduced birth weights respectively,before and after adjustment for major confounders including maternal age,education,parity,occupational exposure to aromatic hydrocarbons,passive smoking,prepregant BMI,infant gestational age and sex However,when EPHX1 and GSTT1,GSTM1 polymorphisms were considered jointly,significant reductions of newborn birth weights were observed among women,with combined genotype of heterozygous variant type EPHX1 and deletion type GSTT1,compared with those among women with combined genotype of homozygous wild type EPHX1 and present type GSTT1;and they were also observed among women with combined genotype of heterozygous variant type EPHX1 and deletion type GSTM1,compared with those among women with combined genotype of homozygous wild type EPHX1 and present type GSTM1 [Conclusion]The association supported speculation regarding the importance of further assessing the role of genes and gene interactions of EPHX1,GSTT1 and GSTM1 in relation to birth weight and offered a new research model to evaluate reproductive toxin in relation to etiology of adverse reproductive outcome