2型糖尿病患者载脂蛋白B基因型频率分布的初步探讨

Preliminary Exploration on Relationship between the Polymorphism of Apolipoprotein B (ApoB) Gene and Type 2 Diabetes Mellitus(DM)

目的：研究载脂蛋白Ｂ（ＡｐｏＢ）基因多态性在本地区２型糖尿病患者中的分布及其与血脂的关系。方法：运用聚合酶链反应一限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）方法检测４１例２型糖尿病患者及６０例正常对照者的ＡｐｏＢ基因型。结果：两组Ｘ＋和Ｅ－等位基因频率分别为０．０８５，０．０１７和０．１１，０．０３３（Ｐ＜０．０１）。糖尿病组血脂总胆固醇（ＣＨ）、甘油三脂（ＴＣ）。低密度脂蛋白胆固醇（ＬＤＬ一ｃｈ）水平均高于对照组（Ｐ＜０、０１）；而高密度脂蛋白胆固醇（ＨＤＬ一ｃｈ）水平低于对照组（Ｐ＜０．０５）。结论；ＡｐｏＢ的Ｘ＋和Ｅ－等位基因可能是２型糖尿病和高脂血症的一个危险因子。

Objective: To investigate the relationship getween the polymorphism of apolipoproteinB (ApoB) gene and type 2 diabetes mellitus (DM) and its serum lipid changes. Methods: The ApoB genetype in 41 type 2 diabetes mellitus and 60 normal control subjects were determined by polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP). Results: The frequencies of X+ and E- alleies in these two groups were respectively 0. 085, 0. 017 and 0. 11, 0. 033 (P < 0. 01). The levels of serum cholesterol (CH), triglyceride (re) and low density lipopro tein - cholesterol (LDL - ch) in type 2 DM patients were significantly higher than control subjects (p < 0. 01), and the levels of high density lopoprotein - cholesterol (HDL - ch) were lower than control (P < 0. 05). Conclusion: The results suggest that X+ and E- alleies probably serve as risk factors for type 2 DM and hyperlipidendas.