黄豆酱多肽原液脱色及抗氧化活性研究

Decolorization and antioxidant activity of soybean paste peptide solution

为去除黄豆酱中的有色物质以利于对生物活性肽的分离纯化及其活性的研究,利用活性炭对黄豆酱水溶液进行了脱色及抗氧化活性研究。在单因素试验的基础上,利用正交试验进行工艺优化,得到的最佳脱色条件为活性炭用量6%,脱色温度35℃,脱色时间40min,溶液pH值为5,脱色率为59.85%,肽存留率为66.35%。脱色液抗超氧阴离子活力270.43U/L,铜离子还原能力7980μmol/L,抑制羟自由基能力820.43U/mL,SDS-PAGE电泳图谱中证实了脱色液冻干粉中多肽和低聚肽的存在。活性炭对黄豆酱多肽原液脱色效果较好,肽存留率及抗氧化活性均较高。该研究为黄豆酱多肽的进一步分离纯化及抗氧化活性研究奠定了实验基础。

In order to separate and purify the bioactive peptides from soybean paste and evaluate its antioxidant activity, the decolorization effects of activated carbon on soybean paste and the antioxidant activity of decolorized soybean paste aqueous solution were investigated. Based on a sin-gle-factor test method, an orthogonal experiment was designed. The results show that the proper decolorizing conditions were activated carbon 6%, decolorizing temperature 35℃, decolorizing time 40min and pH value 5, Under above conditions, the decolorization rate was 59.85% and the peptide retention rate was 66.35%. The superoxide anion radical scavenger activity of decolorized solution was 270.43U/1, the cupric reducing antioxidant ca- pacity was 7980μmol/L and the hydroxyl radical scavenger activity was 820.43U/ml. SDS-PAGE electrophoresis proved the presence of polypeptides and oligopeptides in the lyophilized powder of decolorized solution. In conclusion, activated carbon could decolor soybean paste peptide aqueous solution effectively with high peptide retention rate and antioxidant activity. This study provides experimental basis for further researches about the separation, purification as well as antioxidant activity of soy paste peptides.