摘要
以黑曲霉(Aspergilus niger)CICC41254为出发菌株,进行紫外线诱变。初筛使用透明圈法,从果胶平板上的突变菌株中,挑取了26株透明圈与菌落直径比值显著大于出发菌株的突变株。将这些突变株进行三角瓶固体发酵复筛,最后筛选出1株高产果胶酶的突变株CICC41254-D8。突变株CICC41254-D8经斜面传代培养了5代,产酶遗传特性稳定,其果胶酶活力最高可达1156.8U/g干基,比出发菌株酶活力(750.0U/g)提高了54.24%。
Aspergilus niger CICC41254 was treated by ultraviolet radiation for the development of high pectinase-producing strain. Transparent circle method was used for preliminary screening, by which 26 mutant strains were selected with higher ratio of transparent circle diameter to colony diame-ter on pectin plate media. These mutant strains were re-screened by solid state fermentation in flasks, and one strain named CICC41254-D8 was se-lected with high pectinase-producing ability. CICC41254-D8 was incubated on slant through 5 generations. It was showed that the genetic expression of pectinase was stable. The pectinase activity could reach 1156.8U/gdry bionass which was 54.24% higher than that of the original strain （750.0U/g）.
出处
《中国酿造》
CAS
2012年第6期134-137,共4页
China Brewing
基金
河南城建学院科研基金项目(2010JYB013)
关键词
黑曲霉
果胶酶
紫外诱变
酶活力
固体发酵
Aspergillus niger
pectinase
UV mutagenesis
enzyme activity
solid state fermentation
