单核细胞趋化蛋白-1启动基因多态性与狼疮肾炎相关性的研究

The study of a polymorphism in the monocyte chemoattractant protein 1 promoter gene with lupus nephritis

目的研究单核细胞趋化蛋白-1(MCP-1)-2518位点功能多态性在山东省汉族群体系统性红斑狼疮(SLE)及狼疮肾炎(LN)中的表达。方法通过聚合酶链反应-限制性片段长度多态性分析检测72例SLE病人及59例健康体检者(对照组)MCP-1基因多态性。结果对照组A/A型基因型频率显著高于SLE患者组(P=0.001),A/G型(P=0.024)、G/G型(P=0.020)多见于SLE患者。与无狼疮肾炎SLE患者相比,患狼疮肾炎的SLE患者较常见基因型为A/G(P=0.024)和G/G(P=0.015)型基因型。相反,无狼疮肾炎的SLE患者65%基因型为A/A型,而患狼疮肾炎的SLE患者仅19%基因型为A/A(P=0.000)。结论山东省汉族群体患SLE的患者MCP-1基因型为A/G或G/G者易发生狼疮肾炎。

Objective ： To examine the role of a functional monocyte chemoattractant protein I （ MCP-1 ） polymorphism at position-2518 in systemic lupus erythematosus （SLE） and lupus nephritis （LN） in the Han nationality in Shandong Province. Methods： MCP-1 genomic variants in 72 SLE patients and 59 controls were detected by polymerase chain reaction （PCR） followed by restriction enzyme-fragment analysis. Results： The frequency of the A/A genotype was significantly higher in controls compared with SLE patients （P =0. 001 ） , whereas both A/G （P =0. 024） and G/G （P =0.020） polymorphism were found more frequently in SLE patients. Patients with LN had the A/G （ P = 0.024 ） and G/G （ P = 0.015 ） genotypes more commonly compared with those without LN. In contrast, 65% of patients without LN were genotyped as A/ A, whereas only 19% of patients with LN had this genotype （P =0. 000） . Conclusion： SLE patients with A/G or G/G genotypes of MCP-1 may be at higher risk of developing LN in the Han nationality in Shandong Province.