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锌指蛋白A20在胃癌组织中的表达及其与胃癌病理分期和转移的相关性研究 被引量：1

The expression of zinc finger protein A20 in gastric cancer and correlation of its expression levels to pathological stages and metastases
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摘要 目的探讨锌指蛋白A20在胃癌组织中的表达及其与临床病理分期和远处转移的关系。方法收集2009年1—10月在我院手术切除的42例胃癌及癌旁组织标本,用RT-PCR和Western blotting法分别检测标本中锌指蛋白A20的表达情况。结果胃癌组织中锌指蛋白A20的表达低于癌旁正常组织,Ⅲ期+Ⅳ期胃癌组织锌指蛋白A20的表达显著低于Ⅰ期+Ⅱ期胃癌组织(P<0.05)。肿瘤细胞的分化程度与锌指蛋白A20的表达水平呈负相关,有远处转移的胃癌组织中锌指蛋白A20的表达显著低于无远处转移的组织(P<0.05),有淋巴转移的胃癌组织中锌指蛋白A20的表达显著低于无转移的组织(P<0.05)。但是锌指蛋白A20的表达水平与患者的年龄、性别没有相关性。结论锌指蛋白A20在胃癌组织中表达降低,A20的表达水平与胃癌细胞的恶性程度、分期、远处转移、淋巴转移有关。 Objective To investigate the correlation of the expression levels of zinc finger proteins A20 to the pathological stages and metastases of gastric cancer. Methods The expression of A.20 was detected by RT-PCR and Western blotting in 42 cases of gastric：, cancer and paracancerous tissue samples which were resected in our hospital from January to October 2009. Results The expression of A20 was lower in the cancer tissues than that in the normal paracancerous tissues. 33 ＋ 34 cancer tissues demonstrated lower levels of A20 than the T1 ＋ T2 tissues （ P 〈 0.05 ）. The expression levels of A20 were negatively correlated to the differentiation stages; lower levels of A20 were observed in cases with distant metastases than that observed in cases without distant metaotases （ P 〈 0.05） ; and lower levels of A20 were observed in cases with lymphatic metastasis than that observed in cases without metastases （ P 〈 0.05 ）. However, no correlation of A20 expression levels to the age or gender was observed. Conclusion The expression of A20 decreased in gastric carcinoma tissues, and A20 expression is correlated to the degree of cell differentiation, TNM staing, distant metastasis and lymphatic metastasis.
作者 陈战 涂永久 郭明 戚沛霖 戴益琛
机构地区 解放军第
出处 《临床军医杂志》 CAS 2012年第3期610-613,共4页 Clinical Journal of Medical Officers
基金 福建省厦门市科技基金(编号:KJ2010014)
关键词 胃肿瘤 胃癌 锌指蛋白A20 TNM分期 分化 转移 gastric neoplasm gastric cancer zinc finger protein A20 TNM stage differentiation metastasis
分类号 R735.2 [医药卫生—肿瘤]
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参考文献19

  • 1闰玮,刘家云,苏明权,李晓瑾,郝晓柯.A20在前列腺癌细胞中的表达[J].细胞与分子免疫学杂志,2007,23(3):232-233. 被引量：5
  • 2Dixit VM, Green S, Sanna V, et al. Tumor necrosis factor-alpha induction of' novel gene products in human endothelialcells in- cluding a macrephage-specific chemotaxin [ J ]. J Biol Chem, 1990,265 ( 5 ) : 2973 - 2878.
  • 3Patel VI, Daniel S, Longo CR, et al. A20, a modulator of smooth muscle cell proliferation and apoptosis, prevents and induces re- gression of neointimal hypmplaa[ J]. FASEB J,2006,20(9) : 1418 - 1830.
  • 4Tewari M, Wolf FW, Seldin MF, et aL Lymphoid expression an- dregulation of A20, an inhibitor of programmed cell death [ J ]. J lmmunol, 1995,154 ( ,4 ) : 1699 - 1706.
  • 5Sun SC. Deubiquitylation and regulation of the immune response [ J ]. Nat Rev Immunol, 2008,8 ( 7 ) :501 - 511.
  • 6Vereecke L, Beyaert R, Van Loo G, et al. The ubiquitin-editin- genzyme A20 ( TNFAIP3 ) is a central regulator ofimmunopatholo- gy [ J ]. Trends Immunol, 2009,30 ( 8 ) :383 - 391.
  • 7Song XT, Evel-Kabler K, Shen L, et al. A20 is an antigenpresen- tation attenuator, and its inhibition overcomes regulatory Tcell- mediated suppression [ J ]. Nat Med, 2008,14 ( 3 ) :258 - 265.
  • 8Honma K, Tsuzuki S, Nakagawa M, et al. TNFAIP3 is the target gene of chromosome band 6q23. 3-q24.
  • 9Hoss in ocular adnexal marginal zone B cell lymphoma[ J]. Genes Chromosomes Cane- er,2008,47 : 1 - 7.
  • 10Rinaldi A, Rancoita PM, Bhagat G. Array-CGH identifies both common and subtype-specific genomic aberrations in marginal zone lymphomas[ J]. Blood,2008, 112 : 622.

二级参考文献7

  • 1Dixit VM,Green S,Sarma V,et al.Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin[J].J Biol Chem,1990,265(5):2973-2978.
  • 2Opipari AW Jr,Boguski MS,Dixit VM.The A20 cDNA induced by tumor necrosis factor alpha encodes a novel type of zinc finger protein[J].J Biol Chem,1990,265(25):14705-14708.
  • 3Opipari AW Jr,Hu HM,Yabkowitz R,et al.The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity[J].J Biol Chem,1992,267(18):12424-12427.
  • 4Storz P,Doppler H,Ferran C,et al.Functional dichotomy of A20 in apoptotic and necrotic cell death[J].J Bio Chem,2005,387(1):47-55.
  • 5Ferran C,Stroka DM,Badrichani AZ,et al.A20 inhibits NF-kappaB activation in endothelial cells without sensitizing to tumor necrosis factor-mediated apoptosis[J].Blood,1998,91(7):2249-2258.
  • 6Golovko O,Nazarova N,Tuohimaa P.A20 gene expression is regulated by TNF,Vitamin D and androgen in prostate cancer cells[J].J Steroid Biochem Mol Biol,2005,94(1-3):197-202.
  • 7Malewicz M,Zeller N,Yilmaz ZB,et al.NF-κB controls the balance between Fas and tumor necrosis factor cell death pathways during T cell receptor induced apoptosis via the expression of its target gene A20[J].J Biol Chem,2003,278(35):32825-32833.

共引文献4

同被引文献32

  • 1石永利,吴鸿雁,樊祥山,张明逸,叶庆,冯振卿.上皮-间叶转变相关蛋白在非小细胞肺癌中的表达及其预后意义[J].中国癌症杂志,2011,21(5):342-347. 被引量：12
  • 2Miller J, McLachlan A D, Klug A. Repetitive zinc-binding domains in the protein transcription factor 111 A from Xennpus oocytes[ J]. EMBO J,1985,4(6) :1609-1614.
  • 3Hoovers J M, Mannens M, John R, et al. High-resolution localization of 69 potential human zinc finger protein genes:a number are clustered [ J]. Genomics, 1992,12 (2) :254.
  • 4Berg J M, Shi Y. The galvanization of biology ; a growing appreciation for the roles of zinc[J] Science, 1996,271 ( 5252 ) : 1081-1085.
  • 5Frankel A D,Pabo C O. Fingering too many proteins [ J ]. Cell, 1988, 53(5) :675,.
  • 6Krishnal S S, Majumdarl I, Grishin N V. Structural classification of zinc finger[ J]. Nucleic Acids Res,2003,31 (2) :562-550.
  • 7MacPherson S,Larochelle M ,Tureotte B. A fungal family of transcrip- tional regulators:the zinc cluster proteins [ J]. Microbiol Mol Biol Rev, 2006,70(3 ) :583-604.
  • 8Klug A, Schwabe ： W. Protein motifs 5 : zinc fingers [J]. FASEB 5, 1995,9(8) :597-604.
  • 9Imoto 1, Yuki Yi Sonoda I, et al. Identification of ZASC1 encoding a Krtippel-like zinc finger protein as a novel target for 3q26 amplifica- tion in esophageal squamous cell carcinomas [ J]. Cancer Research, 2003,63 ( 18 ) :63-65.
  • 10Yu T,Chen X, Zhang W, et al. Regulatlon of the potential marker for intestinal cells,Broil ,by ：-catenin and the zinc finger protein KLF4: implications for colon cancer [ J]. J Biol Chem, 2012, 287 ( 16 ) : 3760-3768.

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临床军医杂志
2012年 第3期

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