期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

脑胶质瘤中HINT1基因甲基化状态的研究 被引量：1

Study on methylation of HINT1 gene promoter in glioma
下载PDF
导出
摘要 目的探讨脑胶质瘤中HINT1基因启动子区的甲基化状态。方法采用双酶切联合定量PCR法和MSP法检测70例脑胶质瘤和30例正常脑组织中HINT1基因启动子区域甲基化状况。结果经双酶切后实时荧光定量PCR和MSP法检测,正常脑组织和胶质瘤HINT1基因启动子区域呈非甲基化状态,与性别、年龄和病理分级无明显的相关性。结论脑胶质瘤中HINT1基因启动子区域呈非甲基化状态。 Objective To discuss the promoter methylation status of HINT1 gene in glioma. Methods We adopted the double digestion and the real-time quantitaty PCR method and MSP method to detect tlie methylation of HINT1 gene promoter of tissues. These tissues included glioma tissue of 70 patients and the normal brain tissue of 30 cases. Results The results of the double digestion and fluorescent quantitative real-time PCR and MSP Method demonstrated that HINTI gene promoter was unmethylated state, both of normal brain tissue and glioma tissue. There was no significant correlation between the unmethylated state of HINT1 gene promoter and gender, age, pathological types and pathologic stage. Conclusion HINT1 gene promoter of glioma is in the unmethylated state.
作者 张蓬海 赵天书 王策 李利 董白晶 常英男 王健 汤加斌 刘晓谦
机构地区 哈尔滨医科大学附属第四医院神经外科
出处 《临床神经外科杂志》 CAS 2012年第3期149-152,共4页 Journal of Clinical Neurosurgery
关键词 神经胶质瘤 甲基化 HINT1基因 glioma methylation HINT1 gene
分类号 R739.41 [医药卫生—肿瘤]
  • 相关文献

参考文献24

  • 1Legler J M, Gloeckler Ries L A, Smith M A, et al. RESPONSE: Re:Brain and other central nervous system cancers:recent trends in incidence and morlality[J]. J Natl Cancer Insl ,1999 ,91:2050.
  • 2Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t ( 3 ; 8 ) breakpoint, is abnormal in digestive tract cancers [ J ]. Cell, 1996,84:587.
  • 3Huebner K, Croce CM. FRA3B and other common fragile sites: the weakest links[J]. Nat Rev Cancer,2001,1:214.
  • 4Huebner K, Croce CM. Cancer and the FRA3B /FHIT fragile locus :its'a HIT [J]. Br JCancer ,2003 , 19,88 : 1501 .
  • 5Wang L,Zhang Y,Li H,et al. Hintl inhibits growth and act ivator protein-1 activity in human colon cancer cells [J]. Cancer Res, 2007,67:4700.
  • 6Zhang YJ, Li H, Wu HC, et al. Silencing of Hintl, a novel tumor suppressor gene , by promoter hypermethylation in hepatocellular carcinoma [ J ]. Cancer Lett, 2009,275 : 277.
  • 7Zambelli D, Zuntini M, Nardi F, et al. Biological indicators of prognosis in Ewings sarcoma: an emerging role for lectin galactoside-binding soluble 3 binding protein (LGALS3BP) [ J ]. Int J Cancer,2010,126:41 .
  • 8Yuan BZ, Jefferson AM, Popescu NC, et al. Aberrant gene expre- ssion in human non smallcell lung carcinoma cells exposed to deme- thylating agent 5 -aza-2 - deoxycytidine [ J ]. Neoplasia,2004 ,6 :412.
  • 9Toyota M, Ahuja N, Ohe-Toyota M, et al. CpG island methylator phenotype in colorectal cancer [ J] . Proc Natl Acad Sci USA, 1999.96 : 8681.
  • 10Kanai Y, Ushijima S, Nakanishi Y, et al. Mutation of the DNA methyhransfrase (DNMT) 1 gene in hmnan colorectal cancers [ J]. Cancer Lett ,2003, 192:75.

同被引文献13

  • 1张永彪,褚嘉祐.表观遗传学与人类疾病的研究进展[J].遗传,2005,27(3):466-472. 被引量：49
  • 2Lima CD,D'Amico KL,Naday I,et al. MAD analysis of FHIT,a putative human tumor suppressor from the HIT protein family[J]. Structure 19975(6):763-774.
  • 3Brenner C. Hint,Fhit,and GaIT: function, structure,evolution,and mech- anism of three branches of the histidine triad superfamily of nucleotide hydrolases and transferases[J]. Biochemistry,2002,41 (29) : 9003-9014.
  • 4Zhang YJ,Li H,Wu HC,et al. Silencing of Hint I ,a novel tumor sup- pressor gene ,by promoter hypermethylation in hepatocellular carcino- ma[J]. Cancer Lett, 2009,275 (2) : 277-284.
  • 5Huang H,Wei X,Su X,et al. Clinical significance of expression of Hintl and potential epigenetic mechanism in gastric cancer[J]. Int J Oncol, 2011,38(6) : 1557-1564.
  • 6Yuan BZ,Jefferson AM,Popescu NC,et al. Aberrant gene expression in human non small cell lung carcinoma cells exposed to demethylating agent 5-aza-2"-deoxycytidine[J]. Neoplasia,2004,6(4) :412-419.
  • 7Wang L,Zhang Y,Li H,et al. Hint! inhibits growth and activator protein- 1 activity in human colon cancer cel|s[J]. Cancer Res,2007,67(10) : 4700-4708.
  • 8Ohta M,Inoue H,Cotticelh MG,et al. The FHIT gene,spanning the chro- mosome 3p14.2 fragile site and renal carcinoma-associated t(3 ;8) break- point,is abnormal in digestive tract caneers[J]. Cell, 1996,84(4) :587- 597.
  • 9Pearson JD,De Wald DB,Mathews WR,et al. Aminoacid sequence and characterization of a protein inhibitor of protein kinaseC[J]. J Biol Chem, 1990,265 (8) : 4583-4591.
  • 10Eiske J ,Huber O. The histidine triad protein Hintl triggers apoptosis independent of its enzymatic activity[J]. J Biol Chem, 2006,281 (37) : 27356-27366.

引证文献1

二级引证文献2

临床神经外科杂志
2012年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部