脑卒中后大鼠海马形态学演变趋势及中药的干预作用研究

Hippocampus Morphological Evolution Trend and Interventional Effects of Chinese Medicinal in Rats with Post Stroke

目的:观察脑卒中后大鼠海马的形态学演变趋势及颐脑解郁方对其的干预作用。方法:雄性Wistar大鼠随机分为正常组、假手术组、MCI组、中药组、西药组、PSD组和VD组。采用同种系微栓子体外注入法制备MCI模型;PSD组术后7 d予21 d慢性不可预知的温和应激加孤养复制PSD模型;VD组术后28 d经水迷宫筛选后进入实验。假手术组、中药组及西药组分别予蒸馏水、颐脑解郁方、尼莫地平干预给药4周。于第1、2、4周光镜下分析海马的形态学结构变化。结果:MCI组海马锥体细胞数量显著减少,细胞排列不整齐。PSD组及VD组锥体细胞数量显著减少,胞体不同程度缩小、核固缩、核碎裂、核溶解,细胞排列不整齐,且呈进行性发展。中药组锥体细胞缺失较少,细胞排列整齐,核仁明显,西药组细胞变性有所改善,部分细胞核固缩,不如中药组改善明显。结论:颐脑解郁方可能通过益肾调气的主治功效改善Papez回路的病理改变,阻断病情的进一步发展。

Objective： To investigate the Hippocampus morphological evolution trend and Yinao Jieyu Formula side of their intervention effects after stroke in rats.Methods： Male Wistar rats were randomly divided into normal group,sham operation group of MCI Group,the Chinese Medicines,western medicine group,the PSD group VD group.The same germ-line micro-emboli in vitro injection was prepared by MCI model;the PSD group after 7 d to 21 d of chronic unpredictable mild stress plus solitary housed and PSD model;the VD group after 28 d after water maze screening to enter the experimental.Sham group,the Chinese medicine group and WM group were given distilled water.Yinao Jieyu Formula nimodipine intervention administered four weeks.1,2 and 4 weeks under the light microscope analysis of morphological structural changes in the hippocampus.Results：Hippocampal pyramidal cells in MCI group reduc the irregular arrangement of cells.The number of pyramidal cells in PSD group and VD group reduced significantly,and soma has different level reduced,pyknosis,karyorrhexis and karyolysis.Pyramidal cells arranged irregularly,and got worse.Chinese medicine group showed that pyramidal cell loss less,and arranged regularly,nucleolus can be seen clearly.Degeneration of pyramidal cells in western medicine group improved,pyknosis in some of pyramidal cells,and not as good as Chinese medicine group.Conclusions：YinaoJieyu Formula iPapez loop pathological change and block the progress of the disease by reinforcing kidney and regulating gas primary.