微流体芯片电泳技术对人血清蛋白的快速分离

Rapid Separation of Human Serum Proteins by Microchip Electrophoresis

通过微流体芯片电泳技术分离人血清蛋白,探讨了常见十字形微流体芯片上样品的电动进样与分离过程,分析了在十字芯片上的进样时间和电压设置对后续样品检测和定量的影响。采用的缓冲体系为:100mmol/L H3BO3,50mmol/L NaCl,5%Dextran(以NaOH调至pH 8.3),该缓冲液能够有效分离人血清蛋白中的白蛋白(Albumin)和4种球蛋白(α1-,α2-,β-,和γ-globulin),并且给出了它们在该缓冲体系中的淌度估算范围为5.15×10-5～47.2×10-5 cm2/(V.s)。在芯片上2min之内可以完成进样和分离,相比于常用的毛细管区带电泳,提高了分析速度。

This study focused on the separation of human serum proteins by microchip eleetrophore- sis. It was illustrated the sample injection time and applied voltages significantly affected the final detection and quantitative analysis on a cross microchip. The developed background electrolyte （BGE） was consisted of 100 mmol/L H3BO3, 50 mmol/L NaCI, 5% dextran （pH 8.3 adjusted by NaOH）. Five protein fractions, α1-,α2-, β-, γ-globulins and albumin in human serum, could be separated, moreover, their effective mobilities were estimated in the range of 5.15-47.2 × 10-5 cm2V-1 s-1 in this buffer system. The injection and separation were finished within 2 min in the chip, which was faster than traditional capillary zone electrophoresis.