血管内皮生长因子联合nm23检测在非小细胞肺癌诊治中的意义

Significance of VEGF united with nm 23 detection in diagnosis and treatment of non-small cell lungcancer

目的研究血管内皮生长因子（VEGF）和nm23在非小细胞肺癌（NSCLC）组织中的表达及相互关系，探讨其在NSCLC诊治中的临床意义。方法采用免疫组织化学链酶素抗生物素蛋白．过氧化物酶连结（sP）法检测60例NSCLC及癌旁正常肺组织中VEGF和nm23的表达，结合临床病理因素进行分析。结果VEGF在NSCLC组织中的阳性表达率（63．3％，38／60）显著高于癌旁肺组织（16．7％，10／60）（χ^2=27．22，P〈0．01）。nm23在NSCLC组织中的阳性表达率（53.3％，32／60）则显著低于癌旁肺组织中的表达（88-3％，53／60）（Xz_17．79，P〈0．01）。VEGF的阳性率与淋巴结转移及组织学分级呈正相关（χ^2=15．23、χ^2=7．25，P〈O．05）。nm23与淋巴结转移呈负相关（χ^2=16．93，P〈0．01）。VEGF阳性患者2年生存率明显低于阴性患者（χ^2=5．55，P〈0．05），nm23则相反（χ^2=12．86，P〈0．05）。VEGF与nm23在NSCLC中表达无相关l生（χ^2=1．83，P〉0．05）。结论VEGF和tam23可能与NSCLC的发生、发展有关，检测VEGF和nm23可能有助于对NSCLC生物学行为的判断，对肺癌患者的诊治和预后评估有积极意义。

Objective To detect the expressions of VEGF and nm23 in non-small cell lung cancer （NSCLC） tissues and their relationships , and explore their clinical value in the diagnosis and treatment. Methods Immunohistochemistry （SP method） was used to detect the expressions of VEGF and nm 23 in NSCLC and peritumor tissues. Results The positive expression rate of VEGF in NSCLC （63.3 %,38/60）was significantly higher than in the peritumor tissues（ 16.7 %, 10/60）（χ^2=27.22, P〈0.01）. nm 23 is on the contrary （53.3 % ,32/60）（χ^2=17.79,P〈0.01）. The positive expression of VEGF in NSCLC was positively correlated significantly with the lymph node metastasis and histological classification（P〈0.05）. The positive expression of nm 23 in NSCLC was negative correlated significantly with lymph node metastasis, P〈0.01.2-year survival rate of the VEGF-positive patients was significantly lower than that of VEGF-negative patients, and nm 23 is on the contrary （χ^2=5.55,P〈0.05）.No correlation was found between the expressions of VEGF and nm 23 in NSCLC （χ^2=1.83,P〉0.05） Conelusion The VEGF and nm 23 may be closely associated to carcinogenesis and progression of NSCLC. Detection of VEGF and nm 23 are beneficial to indicate the biologic behavior of NSCLC. It has positive effect on diagnosis and treatment of NSCLC.