肺癌患者外周血中CD＋8CD2-28及CD＋4CDhigh25CDlow127调节性T细胞的表达及其临床意义

Expression and clinical significance of CD＋8CD-28andCD＋4CDhigh25CDlow127 regulatory T cells in peripheralblood of lung cancer patients

目的观察肺癌患者外周血CD＋8CD2-28及CD＋4CDhigh25CDlow127协调节性T（Treg）细胞的表达水平，并对CD[CD；8和CD＋4CDhigh25CDlow127Treg细胞行CD＋8CD2＋28、CD＋8CD2-28及CD＋4CDhigh25CDlow127调Treg细胞的表达率，以60名健康体检者作为对照，分析各指标与肺癌生物学及临床特征之间的关系。结果肺癌组cD嘻C跽T细胞[（58．430±15．749）％]和CD＋4CDhigh25CDlow127Treg细胞[（7．365±2．025）％]表达均分别显著高于对照组的（41．057±15．436）％、（6．648±1．669）％，差异有统计学意义（t=6．102，P〈0．05；t=2．115，P〈0．05）。肺癌组CD＋8CD2＋28T细胞表达[（41．570±15．739）％]低于对照组[（58．700±15．298）％]，差异有统计学意义（t=-6．043，P〈0．05）。上述三个指标的表达一与．性别、年龄及病理学类型无关（P〉0．05）。随着TNM分期增加，CD＋4CDhigh25CDlow127Treg细胞表达逐渐升高，其中Ⅳ期和Ⅲ。期之间差异有统计学意义（t=3．898，P〈0．05）。而CD＋8CD2-28、CD＋8CD2＋28T细胞的表达与临床分期无关（P〉0．05）。CD＋8CD2-28与CD＋4CDhigh25CDlow127Treg细胞的表达无线性相关（r=-0．169，P〉0．05）。结论CD＋8CD2-28、CD＋4CDhigh25CDlow127Treg细胞在肺癌患者外周血呈高表达，CD＋8CD＋28,T细胞呈低表达，这可能是肺癌患者免疫功能低下的原因之一。CD＋8CD-28,与CD＋4CDhigh25CDlow127Treg细胞之间无相关性，但两者联合检测可对了解肺癌患者免疫功能，并为肺癌寻找特异性免疫治疗及预后评价提供新的参考。

Objective To observe the changes of CD＋8CD-28, CD＋8CD＋28 and CD＋4CDhigh25CDlow127 regulate T （Treg） cellsin peripheral blood of lung cancer patients, and to analyze the correlation between CD＋8CD-28 and CDCD＋4CDhigh25CDlow127 Treg cells to reveal the role and clinical significance of them in lung cancer patients, Methods Flow CD＋4CDhigh25CDlow127 Treg ceils in peripheral cytometry was applied to evaluate the level ofCD＋8CD-28,CD＋8CD＋28 and CD＋4CDhigh25CDlow127 Treg blood of 60 untreated lung cancer patients and 60 healthy controls group. The association of each term with clinical features was analyzed. Results The percentage of CD＋8CD-28andCD＋4CDhigh25CDlow127 Treg cells in lung cancer group [（58.430＋15.749） %,（7.365＋2.025） %] was significantly higher than those in healthy group [（41.057±15.436） %,（6.648±1.669） %,（t=6.102,P〈0.05;t=2.115,P〈0.05）], while the percentage of CD＋8CD＋28cells is lower （41.570±15.739）% than that in healthy group [（58.700±15.298） %,（ t=-6.043,P〈0.05）]. No close associations were found between three index and gender, age, and biological characteristics. With the increase of TNM stage, The percentage of CD＋4CDhigh25CDlow127 Treg cells increased gradually, which was remarkably higher in patients with stage 1V than that with stage Ilia （t=-3.898,P〈0.05）. The percentage of CD＋4CDhigh25CDlow127Treg cells was uncorrelated with CD＋8CD-28 cells （r=-0.169,P〉0.05）. Conclusion The higher percentage of CD＋8CD-28andCD＋4CDhigh25CDlow127 Treg cells, the lower percentage of CD＋8CD＋28cells may be the important reasons of immune suppression in lung cancer patients. Though there is no correlation between CD＋8CD-28andCD＋4CDhigh25CDlow127 Treg cells, it is may be helpful to understand immunologic function and it may look for more specific therapy and provide a new reference in the prognosis of lung cancer.