免疫组织化学方法和荧光原位杂交技术检测胃癌组织中Her-2表达的比较

Comparation of immunohistochemistry and fluorescence in situ hybridization for detection of Her-2expression in gastric cancer

目的探讨不同方法检测胃癌中Her-2蛋白表达和基因表达的相关性，寻求胃癌中Her-2检测的有效手段。方法应用免疫组织化学方法（IHC）对68例胃癌进行Her-2蛋白标记，同时采用荧光原位杂交技术（FISH）检测Her-2基因扩增的情况。结果68例胃癌患者中，IHC检测Her-2蛋白阳性12例（17．65％），分别＋＋＋3例，＋＋9例、＋7例和-49例；FISH检测Her-2基因扩增11例（16．17％），与IHC阳性强度相对应的FISH检测结果分别为3例（100％）、8例（88．89％）、0例、0例。Her-2基因扩增与Her-2蛋白表达存在相关性（P〈0．05）。结论胃癌中存在Her-2蛋白的表达和基因扩增，Her-2蛋白在胃癌中的表达呈现明显的异质性。IHC与FISH检测胃癌Her-2的表达差异主要在IHC＋＋组，IHC＋＋有意愿进行Her-2基因靶向治疗的患者建议进行Her-2基因检测。

[Abstract] Objective To investigate the association of Her-2 protein expression and gene expression in gastric cancer by different methods, and to explore the effective approaches of Her-2 in gastric cancer detection. Methods Immunohistochemistry （IHC） was used to mark Her-2 protein in 68 cases of gastri cancer,and fluorescencein situ hybridization（FISH）was used to detect the Her-2geneamplication Results Among68 cases, IHC Her-2 protein in 12 cases （17.65 %） was positive expression, including ＋＋ 3 cases ＋＋ 9 cases, ＋ 7 cases and - 49 cases. By FISH, Her-2 gene in 11 cases （16.17 %） was overexpression. Corresponding to the IHC results, FISH results was 3 cases （100 %）, 8 cases （88.89 %）, 0 cases, 0 cases, respectively. Conclusion Her-2 protein expression and gene overexpression occur in gastric cancer. Her-2 protein expression in gastric cancer shows significant heterogeneity. Her-2 gene overexpression and Her-2 protein expression is correlated （P〈0.05）. The differences of Her-2 expression in gastric cancer by IHC and FISH mostly occur in IHC＋＋ groupe . Suggestion of Her-2 gene test should be given for patients with IHC＋＋ who will be undertaken Her-2 gene therapy.