摘要
目的:建立高效液相色谱法检测复方二氧丙嗪茶碱片中茶碱含量。方法:色谱柱为Kromasil-C18(250 mm×4.6 mm,5μm);流动相为水-甲醇(65∶35);流速为1.0 ml·min-1;检测波长:275 nm。结果:茶碱在0.107～5.335μg·ml-1范围内,与峰面积值呈良好的线性关系,r=0.999 7;平均加样回收率为100.1%,RSD=0.36%(n=9)。结论:方法简便、准确、重复性好,可用于该制剂的含量测定。
Objective： To establish a method for the determination of theophylline in compound dioxopromethazine hydrochloride tablets by HPLC. Method： The column was Kromasil-C18 （250 mm × 4.6 mm ,5μm）. The mobile phase was water-methanol （65： 35）. The flow rate was 1.0 ml · min^-1 with the detection wavelength of 275 rim. Result： The linear range of theophylline was 0. 107- 5. 335 μg · ml^-1, r = 0. 999 7. The average recovery was 100.1% with RSD of 0.36% （n = 9）. Conclusion： The method is simple, accurate and repeatable.
出处
《中国药师》
CAS
2012年第6期824-825,共2页
China Pharmacist
