HPLC法测定复方鱼腥草合剂中绿原酸、黄芩苷的含量

Determination of Chlorogenic Acid and Baicalin in Compound Yuxingcao Mixtures by HPLC

目的:建立同时测定复方鱼腥草合剂中绿原酸、黄芩苷的高效液相色谱法。方法:采用Sinochrom ODS-BP(250 mm×4.6 mm,5μm)色谱柱;流动相为甲醇:0.2%磷酸溶液,进行梯度洗脱;柱温:30℃流速为0.9ml·min-1,检测波长为315 nm。结果:绿原酸在0.1～1.0μg·ml-1范围内线性关系良好(r=0.999 5),平均回收率为100.26%,RSD=0.53%(n=9);黄芩苷在1.8～18.0μg·ml-1范围内线性关系良好(r=0.996 6),平均回收率为100.04%,RSD=0.87%(n=9)。结论:本方法简便易行,能够准确、快速测定复方鱼腥草合剂中的绿原酸和黄芩苷的含量。

Objective ： To establish an HPLC method for the determination of chlorogenic acid and baicalin in compound Yuxingcao mixtures. Method： The Sinochrom ODS-BP（250 mm ×4.6 mm, 5 μm） column was used, methanol： 0.2% H3PO4 was applied as the mobile phase with gradient elution, the column temperature was at 30℃, the detection wavelength was 315 nm and the flow rate was 0.9 ml · min-1. Result： The linear range of chlorogenic acid was 0.1-1.0 μg · ml^- 1 （r = 0.999 7 ） with the average recovery of 100.26% （ RSD = 0.53 %, n = 9）, and that of baicalin was 1.8-18.0 μg · ml^ -1 （ r = 0.998 3 ） with the average recovery of 100.04% （ RSD = 0.87% , n = 9 ）. Conclusion ： The method is simple, rapid and sensitive, and suitable for the content determination of chloro- genic acid and baicalin in compound Yuxingcao mixtures.