摘要
目的:建立血凝仪测定降纤酶效价的方法。方法:以纤维蛋白原为底物,采用血凝仪测定降纤酶对底物的凝固时间,根据对照品的凝固时间及效价,来计算降纤酶的效价。结果:降纤酶浓度在2.5~20 U.ml-1的范围内线性关系良好,回归方程:lgY=2.094 3lgX-0.785 0,r=-0.999 0。平均回收率为97.6%,RSD为1.16%(n=9)。结论:本方法简便、灵敏、准确。
Objective: To establish the potency determination method of defibrase by blood coagulation analyzer. Method: The blood coagulation time was determined by blood coagulation analyzer, and the potency was calculated. Result: The calibration curve was linear within the range of 2.5-20 U ·ml-1. The average recovery was 97.6% with RSD of 1.16% (n = 9), Conclusion: The method was simple, reliable and accurate, and can be applied in the quality control of the preparation.
出处
《中国药师》
CAS
2012年第6期843-845,共3页
China Pharmacist
基金
国家科技支撑计划课题(编号:2008BA2551304-3-2)
关键词
降纤酶
血凝仪
凝血时间
Defibrase
Blood coagulation analyzer
Blood coagulation time