Calpain-10基因多态性与黎族2型糖尿病胰岛素抵抗的关系

Relationship between polymorphism of calpain-10 gene 44 single nucleotide and insulin resistance in type 2 diabetic patients of Li minorities in Hainan

目的研究Calpain-10基因44位点单核苷酸多态性与海南黎族2型糖尿病(T2DM)胰岛素抵抗的关系,探讨Calpain-10与T2DM发病易感性之间的关系。方法选择海南黎族健康查体者54例为正常对照组,T2DM患者46例为T2DM组。测量所有研究对象的身高、体重、腰围、臀围,计算体重指数(BMI)、腰臀比(WHR)。并且空腹抽血测定胰岛素(Fins)、空腹血糖(FPG)。用稳态模型方法,计算胰岛素抵抗指数(IRI)及胰岛β细胞功能指数(HBCI)。对所有研究对象Calpain-10基因UCSNP-44多态性进行基因分型并计算其优势等位基因频率。结果①黎族人正常对照组UCSNP-44 TT基因型占78%,TC型占20%,CC型占2%;T等位基因频率为88%,C等位基因为12%。黎族人T2DM组UCSNP-44 TT基因型占76%,TC型占22%,CC型占2%;T等位基因频率为88%,C等位基因为12%。2型糖尿病组与正常对照组比较,UCSNP-44位点等位基因及基因频率差异无统计学意义(P>0.05)。按BMI≥25 kg/m2、BMI<25kg/m2分别比较,各组之间也无统计学差异(P>0.05)。②T2DM组内UCSNP-44 TT组与TC+CC组BMI及WHR、FPG、FIns、IR、HBCI均无明显差异(P>0.05)。结论本研究未发现Calpain-10基因UCSNP-44位点变异与T2DM及胰岛素抵抗发病相关,提示Calpain-10基因UCSNP-44并非海南黎族人群中T2DM常见相关发病基因。

Objective To study relationship between 44 single nucleotide polymorphism in Calpain-10 gene and insulin resistance in type 2 diabetic（T2DM）patients of Li minorities in Hainan.Methods 46 T2DM patients（T2DM group）and 54 healthy subjects（control group）were enrolled in this study.The height,weight,waist circumference,hip circumference,body mass index（BMI）,waist to hip ratio（WHR）,and the levels of fasting blood insulin（FIns）and fasting plasma glucose（FPG）in both groups were measured.Results In the control group,TT genotype of UCSNP-44 was accounted for 78%,TC-type accounted for 20%,CC-type accounted for 2%,T allele frequency was 88% and C allele was 12%.In the T2DM group,TT genotype was accounted for 76%,TC-type 22%,CC-type 2%,T allele frequency 88%,C allele 12%.Compared with the control group,UCSNP-44 allele and gene frequencies in the T2DM group did not show significant differences（P〈0.05） and no significant difference in BMI≥25kg/m^2 and BMI 25 kg/m^2 between two groups（P〈0.05）.No significant differences in BMI and WHR,FPG,FIns,IR,HBCI between the TT subgroup and the TC＋CCsubgroup in the patient with T2DM（P〈0.05）.Conclusion UCSNP44 sites of calpain 10 gene are not related to type 2 diabetes,calpain 10 gene might not be a related gene for type 2 diabetic patients of Li minorities in Hainan province.