摘要
Thirty-two C-genome specific candidate bacterial artificial chromosome （BAC） clones were successfully screened from the BAC library by four-dimensional PCR method with the primer pairs of 75 simple sequence repeat （SSR） markers located in the nine C-genome linkage groups of Brassica napus. The screened 32 BAC clones have an average insert size of 114.2 kb with a range of 30–190 kb. They are the first set of C-genome BAC clones screened from B. napus genomic BAC library. The average insert size of this set of BAC clones presented that the constructed BAC library had a high quality. This set of BAC clones can be used as markers to identify individual chromosomes of B. napus C-genome.
Thirty-two C-genome specific candidate bacterial artificial chromosome （BAC） clones were successfully screened from the BAC library by four-dimensional PCR method with the primer pairs of 75 simple sequence repeat （SSR） markers located in the nine C-genome linkage groups of Brassica napus. The screened 32 BAC clones have an average insert size of 114.2 kb with a range of 30–190 kb. They are the first set of C-genome BAC clones screened from B. napus genomic BAC library. The average insert size of this set of BAC clones presented that the constructed BAC library had a high quality. This set of BAC clones can be used as markers to identify individual chromosomes of B. napus C-genome.
基金
Supported by the National Natural Science Foundation of China (30671312)
the Natural Science Foundation of Hubei Province(2008CDA083 and 2009CDB191)
the Chenguang Program of Wuhan City(201050231022)
