超声波辅助提取银杏蛋白工艺条件的优化

Optimization of Ultrasonic-Assisted Extraction Technology of Protein from Ginkgo Biloba

以银杏为原料,利用超声波辅助提取银杏蛋白。在单因素试验的基础上,采用Design-Expert软件中的Central-Composite中心组合设计,确定超声波辅助提取银杏蛋白的最佳工艺条件,建立提取的回归数学模型。试验结果表明,曲面回归方程拟合性好,所建数学模型能较准确地预测银杏蛋白溶出量。银杏蛋白的最佳工艺条件为提取温度45℃,pH10,超声波提取时间20min,液料比20mL/g,超声波功率300W。在此试验条件下蛋白溶出量为61.748g/mL。

The text used Ginkgo biloba defatted powder as material to extract protein by ultrasonic wave.At the base of single factor test,the optimum technological conditions were determined by Central-Composite principles in the Design-Expert software with the central composite experimental design;the regressive mathematics model of extraction rate was built.The result showed that the fitting resistance of the surface regression equation was excellent;the mathematical model could accurately predict the dissolution of protein.And the optimal conditions were as follows：extraction temperature 45 ℃,pH 10,extraction time of ultrasonic wave 20 min,liquid to material 20 mL/g,the power of ultrasonic wave 300 W.In these conditions,the dissolution of protein was 61.748 g/mL.