摘要
目的探讨复色1号方对小鼠B16黑素瘤细胞增殖及酪氨酸酶活性的影响。方法采用MTT法测定不同浓度的复色1号方对小鼠B16黑素瘤细胞增殖的影响,多巴速率氧化法测定对酪氨酸酶活性的影响,对其结果进行统计分析。结果复色1号方浓度在0.001～0.01g/l,范围内对小鼠B16黑素瘤细胞的增殖与对照组相比无统计学意义(P>0.05),0.05～0.1g/l浓度范围内对小鼠B16黑素瘤细胞的增殖与对照组相比有显著意义(P<0.05),呈现一定的抑制作用;复色1号方在0.001～0.1g/l浓度范围内对小鼠B16黑素瘤细胞中酪氨酸酶活性的影响与对照组相比,各浓度含药培养基组的A值均有显著性差异(P<0.05),呈剂量依赖性激活作用。在0.001～0.01g/l浓度范围内,复色1号方的最佳激活浓度为0.01g/l。结论复色1号方没有促进小鼠B16黑素瘤细胞增殖的作用;在一定浓度范围内(0.001～0.1g/l),对小鼠B16黑素瘤细胞酪氨酸酶的活性呈剂量依赖型激活作用。
Objective To study the effects of Fuse Formula-1 on cell proliferation and tyrosinase activity of cultured B 16 melanoma ceils. Methods MTT method, DOPA-rate limiting method were employed to measure the cell viability and tyrosinase activity of B16 melanoma cells. And divided into experimental group （Fuse Formula-1）, control group（bland control group）, compared their results. Results Fuse Formula-1 concentration of 0.001 - 0.01 g/1 g/L for B 16 melanoma cells had no effect （P 〉 0.05）, 0.05 - 0.1 g/lg/L of Fuse Formula-1 on B 16 melanoma cells for inhibition （P〈 0.05）; 0.001-0.1g/1 Fuse Formula-lfor B16 melanoma cells, tyrosinase activity was in a dose-dependent activation. Conclusion Fuse Formula-1 had no effect on cell proliferation of B16 melanoma cells, but exhibits a dose-dependent enhancing effects On tyrosinase activity in B 16 melanoma cells.
出处
《中国中医药现代远程教育》
2012年第6期152-154,共3页
Chinese Medicine Modern Distance Education of China
基金
广东省"211工程"三期重点学科建设项目[No:粤发改社(2009)431号]
