期刊文献+

高效液相色谱法测定不同产地葛花中葛花苷的含量 被引量:5

HPLC determination of kakkalide in Puerariae lobata Ohwio from different areas
下载PDF
导出
摘要 目的建立高效液相色谱法测定葛花中葛花苷含量方法。方法采用依利特Hypersil ODS(4.6 mm×150mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸(17∶83),流速为1mL.min-1,柱温为35℃,检测波长:265nm,进样量:10μL。结果葛花苷在0.102 4~1.229mg.mL-1内与峰面积线性关系良好,平均回收率为100.9%,RSD为1.7%。结论所建立之方法准确可靠、灵敏度高、专属性强,可用于测定葛花中葛花苷的含量。 Objective To establish an HPLC method for the determination of kakkalide in Pueraria lobata Ohwi. Meth- ods Hypersil ODS (4. 6 min×150 mm, 5 μm) was used with acetonitrile-1% phosphoric acid solution as the mobile phase. The flow rate was 1.0 mL·min 1 and the column temperature was 35℃. The detection wavelength was 265 nm and the injection volume was 10μL. Results The linear range of kakkalide was 0. 102±1. 229 mg·mL-1. The average recovery was 100.9%, with RSD was 1.7%. Conclusion The method is reliable, accurate and specific with good reproductivity and can be used for the content determination of kakkalide in Pueraria lobate Ohwi.
作者 华卡 黄宇
出处 《中南药学》 CAS 2012年第6期435-437,共3页 Central South Pharmacy
关键词 高效液相色谱法 葛花 葛花苷 HPLC Pueraria lobata Ohwi kakkalide
  • 相关文献

参考文献8

二级参考文献40

共引文献49

同被引文献38

引证文献5

二级引证文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部