变蛋中微量铅的催化示波极谱法测定

DETERMINATION OF THE MICRO-AMOUNT OF LEAD IN PRESERVED EGG BY CATALYTIC OSCILLOPOLAROGRAPHY

用催化示波极谱法测定变蛋中微量铅 ,提出在HCl-酒石酸 -KI -抗坏血酸体系中 ,用催化示波极谱法测定变蛋中微量铅。在此体系中 ,Pb(ⅠⅠ )于 -0 54V处产生一个非常灵敏的吸附催化波。铅含量在 0 0 2 5～ 3 μg·mL-1 范围内波高与浓度呈良好线型关系 ,相关系数r为 0 9976,检测限为0 0 2 5μg·mL-1 。样品经HNO3 -HClO4消化和分解 ,蒸至近干后 ,再用HCl溶解 ,在HCl-酒石酸 -KI体系中测定 ,回收率为 ( 98± 2 ) %。此方法具有灵敏、准确、快速等特点 ,适用于变蛋及其他食品。

there is no report about the method of determination of micro lead in preserved egg by catalytic oscillmeter polarography in before refernce It presents a catalytically oscillopolarographyographic preserved egg in HCltartaric acidHClO 4,in this system a very sensitive absorbing catalytic wave is produced by lead(Ⅱ) at -0 54 V in the region of the amount of lead from 0 025～3 μg·mL -1 ,there is a well linear relation of the peak with concentration of lead(Ⅱ) The detection limit is 0 025μg·mL -1 After preserved is decomposed by HNO 3HClO 4 added by standard resolution The average relyle rate of lead is (98±2)% This method is of sensitation,accuracy and rapidly,it may be used to determine the microamount lead in other food , human being hair and so on. [Key words] preserved egg; lead; catalytic oscillometer polargraphy; digestiveof two groups were significantly different ( P <0.01).Only 14.2% and 3.33% eggs could develop into 2 cell stage embryos after microinjecting antisense α and antisense β( P <0.01).The result suggests that 1 cell stage eggs can’t divide into 2 cell stage after long time treatment of TPA and antisense oligodeoxynucleotide of PKC(α、β) can inhibit the division of 1 cell stage eggs.