摘要
目的探讨诺氏梭菌在小鼠乳癌模型体内应用的靶向性和安全性。方法制备小鼠乳癌模型。将减毒诺氏梭菌经尾静脉注射入模型小鼠体内,在注射后的第1、2、4、7天分别处死小鼠,取其肿瘤组织及肝、肾、肺匀浆做厌氧培养,观察减毒诺氏梭菌在小鼠肿瘤组织和各器官中的分布。取注射后第2天瘤体及上述器官制作组织切片,分别用苏木精-伊红及革兰染色,观察瘤体中央坏死区大小及细菌的分布。将减毒诺氏梭菌经尾静脉注射入正常小鼠体内,观察小鼠一般情况及处死后细菌在肝、肾、肺中的分布,评价其在小鼠体内应用的安全性。结果荷瘤小鼠经尾静脉注射减毒诺氏梭菌后,细菌在瘤体中持续大量繁殖;各器官中有少数细菌分布,随时间的延长逐渐减少甚至被全部清除。组织学观察显示实验组小鼠肿瘤中央坏死区大于对照组。正常小鼠注射细菌后一般情况未见异常,7d后各器官培养未见细菌。结论减毒诺氏梭菌对小鼠乳癌具有良好的靶向性,并具有一定的溶瘤作用;其在实验小鼠体内应用有较高的安全性。
Objective To investigate the target and safety of C. novyi-NT used in a mouse model with breast cancer. Methods A breast cancer model in mouse was created. The attenuated C. novyi-NT were injected into the model mice through ve na caudalis, the mice were killed on days l, 2, 4, and 7 after the injection. The tumor tissue and homogenate of liver, kidney and lung were taken for anaerobic culture, the distribution of attenuated C. novyi-NT in tumor tissue and each organ of the mice were observed. The tumors and the above mentioned organs were collected, stained by hematine-eosin or Gram stain, to make histologi- cal sections, the zone of necrosis in tumor centre and distribution of the bacteria were observed. The attenuated C. novyi-NT were injected intravenously into normal mice through vena caudalis. The safety of C. novyi-NT in normal mice was observed that inclu- ded the general condition and bacteria distribution in liver, kidney and lung. The safety of application of attenuated C. novyi-NT in the mouse was valuated. Results A great number of C. novyi-NT to multiply in the tumor after injection of attenuated C. novyi- NT, and a few of becteria were seen in each organ, which were decreased along with the extension of time, and even cleared. His- tologically, the zone of necrosis in the centre of the tumor was larger than that in the normal control. In normal mice, the general condition was not changed after injection of the bacteria, and the result after seven-day bacterial culture of each organ was negative. Conclusion Attenuated C. novyi-NT has a favourable target toward the mice with breast cancer, and with a certan oncolysis, which has a relative safety in experimental mice.
出处
《青岛大学医学院学报》
CAS
2012年第4期327-329,333,共4页
Acta Academiae Medicinae Qingdao Universitatis
