消毒剂-磺胺耐药基因的检测

DETECTION OF DISINFECTANT-SULFADIAZINE-RESISTANCE GENE qacE△1-sul1

目的检测临床分离的革兰阴性杆菌菌株Ⅰ类整合子消毒剂-磺胺耐药(qacE△1-sul1)基因的携带情况。方法采用聚合酶链反应技术,对311株革兰阴性杆菌临床分离菌株(分别为大肠埃希菌96株,肺炎克雷伯菌肺炎亚种71株,铜绿假单胞菌64株,鲍氏不动杆菌61株,阴沟肠杆菌19株)进行qacE△1-sul1基因的检测。结果 311株革兰阴性杆菌中180株qacE△1-sul1基因阳性,大肠埃希菌、肺炎克雷伯菌肺炎亚种、铜绿假单胞菌、鲍氏不动杆菌、阴沟肠杆菌qacE△1-sul1基因阳性率分别为69.8%、53.5%、37.5%、75.4%、26.3%。大肠埃希菌和鲍氏不动杆菌qacE△1-sul1基因阳性率高于肺炎克雷伯菌肺炎亚种、铜绿假单胞菌和阴沟肠杆菌,差异有显著意义(q=3.019～6.129,P<0.05)。结论临床分离菌株qacE△1-sul1基因高携带率可能是目前医院感染的高发因素之一,临床应重视消毒剂的合理使用,并定期了解医院细菌耐消毒剂的情况。

Objective To detect the distribution of antiseptic sulfadiazine-resistance gene qacEAl-sull in gram-negative bacteria isolated from chnic. Methods A total of 311 gram-negative strains, including 96 strains of Escherichia coli,71 strains of Klebsiella pneumoniae,64 strains of Pseudomonas aeruginosa,61 strains of Acinetobacter baumannii, and 19 strains of Enterobac- ter, isolated clinically were detected by polymerase chain reaction （PCR） for qacE△l-sull gene. Results In the 311 strains, 180 were positive for qacE△l-sull gene （57. 9%）, the positive rates of qacEA1 su11 gene in E. coil, K. pneumoniae, P. aeruginosa, A. baumannii, and E. cloacae were 69.8 %, 53.5 %, 37.5 %, 75.4 % and 26.3 %, respectively. The positive rates of qacE△ 1-sull gene of E. coli and A. baumannii were higher than K. pneumoniae, P. aeruginosa and E. cloacae, the differences were statistically significant （q=3. 019-6. 129 ,P〈0.05）. Conclusion The high percentage of qacE△l sull gene is likely to be one of the factors contributing to higher infection now in hospital. Rational use of antiseptic should be thought highly, and the condition concerning bacteria-resistance to disinfectant should be regularly investigated.