摘要
目的:观察舒肝颗粒对活化大鼠肝星状细胞表达转化生长因子TGF-β1的影响,以探讨舒肝颗粒对大鼠肝星状细胞活化的影响及相关机制,为舒肝颗粒临床应用提供进一步理论依据。方法:用空白对照组,0.01、0.02、0.04 mg/mL的舒肝颗粒分组处理活化的HSC 24 h后,用放射免疫法测定各组HSCTNF-α表达情况,荧光定量PCR法测定各组HSC TGF-β1表达情况。结果:用荧光定量PCR法测各组TGF-β1的表达情况:空白对照组、舒肝颗粒0.01 mg/mL组、舒肝颗粒0.02 mg/mL组及舒肝颗粒0.04mg/mL组TGF-β1表达水平通过计算2-△△Ct值分别为:(1.00±0.09)、(0.71±0.08)、(0.55±0.07)、(0.44±0.03),且组间差异有统计学意义(P<0.01)。结论:舒肝颗粒可通过抑制HSC TGF-β1的表达,发挥其抗纤维化作用。
Objective: To research the affect of shu-gan granule on expression of TGF-β1 on activation of Hepatic Stellate Ceils in Rat activated invitro, investigate the main mechanism of shu-gan granule in protection of liver fibrosis. Methods: The experiment was divided into the blank control group, shu-gan granule 0. 01 mg/mL group, shu-gan granule 0. 02 mg/mL group and shugan gran- ule 0. 04 mg/mL group. HSC was intervented in vitro for 24h, then detected TGF-β1 expression by fluorescence quantitative PCR. Re- suits: The numerical of 2-△△Ctof control group and each drug intervention group were: (1.00 ± 0. 09), (0. 71 ± 0. 08), (0.55 ±0. 07), (0. 44 ±0. 03), and there were statistical significance among them (P 〈0. 01 ) . Conclusion: Shu-gan granule can resist hepatic fibrosis through inhibiting the expression of HSC TGF-β1.
出处
《成都中医药大学学报》
2012年第2期37-42,共6页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家人事部留学回国人员基金(编号:030079)
四川省卫生厅资助项目(编号:川卫125号)