LSA、ADA在鼠肝细胞癌变过程中的表达与动态改变

Expression and Alterations of Lipid Associated Sialic Acid and Adenosine Deaminase during the Development of Rat Hepatoma

探讨脂质结合唾液酸 (LSA)、腺苷脱氨酶 (ADA)在肝癌诊断中的价值。方法 :以化学致癌剂制作鼠肝癌模型 ,对鼠肝细胞在癌变不同阶段血清中的 LSA、ADA变化进行实验研究。结果 :LSA在鼠肝癌发生过程中 ,呈持续增加趋势 ,其浓度在癌前病变鼠和肝癌鼠中非常明显地高于对照鼠 (P<0 .0 1 ) ;ADA在肝细胞变性鼠和癌前病变鼠中明显异常 (P<0 .0 1 ) ,其活性增加数倍到十几倍 ,但在鼠肝完全发生癌变后 ,ADA活性则趋于正常。结论 :ADA在肝细胞损伤时较敏感 ,L SA则可望作为肝细胞癌变的标志物。

Objective To explore the diagnositic values of lipid associated sialic acid (LSA)and adenosine deaminase(ADA)in hepatocellular carcinoma.Method The alteration of serum LSA concentration and serum ADA activity were investigated in Sprague Dawley (SD) rats inducing with 2 Fluorenyl acetamide during the development of rat hepatoma.Results The LSA concentrations in sera of rats were everlastingly increased in the development of hepatoma,and the values was significantly higher( P <0 01) in percancerous rats and hepatoma rats than that of the control rats.The activities of serum ADA were markly increased ( P <0 01)in the hepatocytes with denaturation and precancerous lesion, and the activities in rats with hepatocyte denaturation or precancerous lesion were increased dozen times than that in the control group.Conclusion The abnormal alterations of LSA and ADA are markers for liver disease.ADA is more sensitive to hepatocellular lesion and LSA may be tumor marker for hepatocellular carcinoma.