血清叶酸与维生素B12的快速联合检测及其临床意义

Rapid Combined Assays and Clinical Significance of Folate and Vitamin B 12 in Serum

应用免疫化学发光技术联合检测人血清的叶酸和维生素 B12 在各种疾病时 (尤其是巨幼细胞性贫血 )的含量变化 ,并对其临床意义进行探讨。方法 :对该院 2 0 0例患者及 50例正常健康人 ,分别用AXSYM全自动免疫分析仪 ,以微粒子酶免分析法 (MEIA)测定 Vit B12 ,以离子捕捉免疫分析法 (ICIA)测定叶酸。并对数据统计分析。结果 :巨幼贫组 48例全部低于正常水平 ,有极显著性意义 (P<0 .0 5)。白血病、恶性肿瘤组血清叶酸低于正常 (P<0 .0 1 )。结论 :叶酸与 Vit B12 的快速测定对贫血与白血病等病人有很重要的临床意义 ,尤其是巨幼贫诊断的可靠指标。

Objective To study the levels of folate and vitamin B 12 in patients(special for megaloblastic anemia).Methods Microparticle Enzyme Immunoassay(MEIA) and Ion Capture Immunoassay(ICIA) of Abbott Axsym System were used to quantitatively detect the folate and vitamin B 12 in serum of 200 patients and 50 normal controls.Results The 48 patients with megaloblastic anemia were significantly lower than that in normal controls (P <0 05).The Folate level of the patients with leucocythemia and malignancy was also lower( P <0 01).Conclusion It is important significance to rapidly combinedly detect the human serum levels of folate and vitamin B 12 in anemia(special for megaloblastic anemia),leucocythemia patients.