PCR扩增16SrRNA基因检测幽门螺杆菌的特异性研究

Study on the Specificity of 16S rRNA Gene for Detection of Helicobacter Pylori

为了评估 PCR扩增 1 6S r RNA基因检测幽门螺杆菌的特异性 ,对 Hp和非 Hp临床分离菌株、胃活检组织、胃黏液、大便、淋巴细胞及其它非胃组织进行了 Hp的检测。 49株 Hp均出现 1 0 9bp特异扩增条带 ,而 7株非 Hp菌株为阴性 ;77份胃活检组织中 ,细菌培养阳性 48份 ,PCR阳性 50份 ;550份吞线取样的胃黏液标本 PCR扩增 Hp阳性 2 61份 (47.5% ) ;2 0份淋巴细胞、2 0份大便、5份乳房组织、4份肌肉组织、7份肺组织、2份脾组织和 3份肝组织标本 ,PCR扩增均阴性。结果显示 ,PCR法扩增 1 6S r RNA基因有较高的特异性 ,可用于临床标本 Hp的检测。

In order to evaluate the specificity of 16S rRNA gene PCR,examined Helicobacter pylori(Hp) and non Hp clinical isolates,gastric biopsy,gastric secretion,fece,lymphocyte and other non gastric tissue specimen for detection of Hp.All forty nine Hp strains produced a 109 bp amplified band.But seven non Hp strains were PCR negative.Of 77 gastric biopsy,48 were positive by culture and 50 were positive by PCR.261(47 5%)of 550 gastric secretion samples with self made sampling tool were Hp positive by PCR.20 lymphocyte,20 fece,5 breast.4 myoideum,7 lung, 2 spleen and 3 liver tissue samples were detected for Hp by PCR.All of these specimens were negative.This study shows that the 16S rRNA gene PCR possess higher specificity and can be used to detect Hp from clinical specimens.