格列美脲对大鼠下颌骨成骨细胞增殖分化及矿化的影响

Effects of glimepiride on proliferation,differentiation and mineralization of rat mandibular osteoblasts

目的:研究格列美脲对大鼠下颌骨成骨细胞增殖、分化及矿化的影响。方法:原代培养分离下颌骨成骨细胞,将细胞接种于96孔板中,分两组:5.5mM葡萄糖(5.5mMG)(生理浓度葡萄糖)组,5.5mMG+10μmol/L格列美脲组,继续培养7d,1)MTT法检测大鼠下颌骨成骨细胞的增殖;2)生化法测定7d碱性磷酸酶(ALP)活性;3)Western blots检测Ⅰ型胶原(ColⅠ)的表达;4)RT-PCR检测骨钙素(OCN)的表达。结果:格列美脲能显著促进成骨细胞的增殖,ALP活性,ColⅠ的蛋白和OCN的mRNA的表达。结论:格列美脲能促进大鼠下颌骨成骨细胞增殖、分化及矿化。

Objective： To investigate effects of glimepiride on proliferation, differentiation and mineralization of rat mandibular osteoblasts. Methods： Primary osteoblasts were isolated and cultured. Then the cells were placed in an osteogenic medium, with or without glimepiride （10p M）. Cell proliferation was determined by MTT assay..Supernatant assay was used for measuring alkaline phosphatase （ALP） activity. Western blot analysis was used for determining Col I expression. RT-PCR analysis was used for determining OCN mRNA level. Results： Glimepiride stimulated rat osteoblast proliferation, ALP activity, Col I expression and OCN mRNA expresson.Conclusion： glimepiride improved the proliferation, differentiation and mineralization of osteoblasts in rats.