IL-8在子宫内膜异位症在位内膜的表达

Expression of interleukin-8 in endometriosis eutopic endometrium

目的:探讨白介素-8(interleukin-8,IL-8)在子宫内膜异位症(endometriosis,EMT)患者的在位子宫内膜中的表达水平及其在EMT发病中的作用。方法:采用免疫荧光方法与Western Blot检测并比较IL-8在EMT患者在位内膜和正常子宫内膜中的表达水平。结果:(1)免疫荧光检测结果显示IL-8在内膜腺体和间质细胞中均有表达,主要定位于内膜腺体的腺泡浆。正常子宫内膜分泌期IL-8的表达(26.577±3.202)高于增生期(17.204±1.905)(P<0.05);EMT在位内膜分泌期IL-8的表达(49.122±5.390)高于其增生期(38.506±2.744)(P<0.01);EMT在位内膜IL-8的表达高于正常子宫内膜(P<0.01);(2)Western Blot结果显示:正常子宫内膜分泌期IL-8的表达(0.346±0.035)与其增生期(0.076±0.015)相比,差异有统计学意义(P<0.01);EMT在位内膜分泌期IL-8的表达(0.659±0.043)高于其增生期(0.437±0.0428)(P<0.01);EMT在位内膜IL-8的表达高于正常子宫内膜(P<0.01)。结论:IL-8可能在病变中起重要作用。

Objective ：To investigate the role of IL-8 in the eutopic endometriosis. Methods： Immunohistochemistry was used to detect the protein expression of IL-8 in the eutopic endometrium, with the endometrium of nomal fertile women as control. Results： Immunofluorescence： All tissue sections have expression of IL-8 in both gland and stroma,but the expression mainly located in gland. The showed no difference between the（14.743±2.095） and （16.183±3.036） （P〉0.05）. In normal endometrium, expression of IL-8 was significantly higher in proliferative phase （17.204±1.905） than that in secretory phase（26.577±3.202）. The expression level of IL-8 in secretory phase（49.122±5.390） was higher than that in proliferative phase（38.506±2.744） （P〈0.01）. Compared with the normal endometrium, the expression level of IL-8 in EMT eutopie endometrium phase was increased （P〈0.01） in both secretory phase and proliferative phase. Western Blot： In normal endometrium,expression of IL-8 was significantly higher in proliferative phase （0.076±0.015）than that in secretory phase（0.346±0.035）. The significant difference ex- isted between secretory phase （0.659±0.043） and proliferative phase（0.437±0.0428） in EMT eutopie endometrium（P〈0.01）. Cornpared with the normal endometrium, the expression level of IL-8 in EMT eutopic endometrium was increased（P〈0.05, P〈0.01） in both secretory phase and proliferative phase. Conclusion： IL-8 may play an important role in the pathogenesis of endometriosis.