补肾方骨青颗粒对佐剂性关节炎大鼠的干预和影响软骨表达酸敏感离子通道3的实验研究

GuQing granules can treat the adjuvant arthritis rat and regulate the acid-sensitive ion channel

目的:观察补肾方骨青颗粒对佐剂性关节炎(adjuvant arthritis,AA)大鼠的干预作用及对软骨酸敏感离子通道3(acid-sensitive ion channel 3,ASIC3)表达的影响。方法:将大鼠随机分成空白对照组、AA模型组、阳性对照药组(阿司匹林50mg/kg)、骨青颗粒治疗剂量组(8.4 g生药/kg)、骨青颗粒高剂量组(16.8 g生药/kg)。除空白对照组外其余各组大鼠左后足趾皮内注射完全弗氏佐剂(complete Freund’s adjuvant,CFA)诱导AA,致炎后第10天起各组灌胃给予相应药物,连续给药10 d。记录大鼠体重足趾容积和关节炎评分,实验结束后,光学显微镜观察大鼠踝关节病理变化,用RT-PCR和Western blot分析大鼠膝关节软骨细胞中ASIC3 mRNA及蛋白表达。结果:经治疗后,骨青颗粒能明显抑制AA大鼠踝关节的肿胀,显著降低AA大鼠关节软骨细胞ASIC3 mRNA及蛋白的表达,效果与阳性对照药物阿司匹林无显著性差异。结论:骨青颗粒可减轻佐剂性关节炎大鼠的关节炎症,保护关节软骨,并能下调软骨ASIC3的表达。

Objective：To observe the curative effect of GuQing granules on the adjuvant arthritis rats,and detect whether GuQing granules could regulate the expression of acid-sensitive ion channel 3（ASIC3） in articular cartilage. Methods：The rats were randomly divided into five groups：blank control group,adjuvant arthritis model group,positive control group （aspirin 50 mg/kg）,GuQing granules group of thearapeutic dose （8.4 g crude drug/kg）,GuQing granules group of high dose （16.8 g crude drug/kg）. Except the rats in blank control group,all the rats were injected intradermally with complete Freund＇s adjuvant （CFA） at left toes to induce adjuvant arthritis model. Ten days after injection, all rats of each group were administration with corresponding drugs for 10 days. The weight, volume of right foot and arthritis scores of rats were recorded., the pathological changes of rat ankle joint were observed by optical microscope, and the mRNA and protein expression of ASIC3 in rat knee cartilage were analyzed by RT-PCR and Western blot. Results：GuQing granules significantly inhibited the paw swelling of rats ankle after treatment, the mRNA and protein expression of ASIC3 in knee cartilage decreased after GuQing granules treatment. There was no significant difference between the positive control aspirin group and GuQing granules treatment group. Conclusion：GuQing granules can reduce the inflammation of adjuvant arthritis rats, protect articular cartilage and down-regulate the expression of ASIC3.