Mcl-1在宫颈癌组织中的表达及临床意义

The expression and clinical significances of Mcl-1 in cervical cancer

目的:探讨Mcl-1在宫颈癌的表达水平及其与临床病理特征的关系。方法:应用qRT-PCR技术检测20例宫颈癌组织及癌旁正常组织Mcl-1mRNA水平,同时应用免疫组织化学技术检测45例宫颈癌组织及正常宫颈组织Mcl-1表达水平,分析其表达与临床病理特征的关系。结果:宫颈癌组织Mcl-1mRNA表达水平明显高于正常宫颈组织(P<0.05),Mcl-1在宫颈癌组织中的表达阳性率为53.3%,明显高于正常宫颈组织的阳性率11.1%(P<0.05),其表达与病理分级、肿瘤大小及淋巴结转移有显著相关性(P<0.05)。结论:Mcl-1的表达与宫颈癌的发生、发展有关,可作为宫颈癌诊断及临床监测的分子标志物。

Objective：To investigate the expression profile of Mcl-1 in cervical cancer and its relationship with the clinical pathological characteristic,and to assess its clinical significance. Methods：Mcl-1mRNA expression both in cervical cancer and in corresponding normal tissue was detected by reverse transcription fluorescent quantitation polymerase chain reaction method. Mcl-1 protein expression was detected by immunohistochemistry method. The correlation of Mcl-1 expression and clinicopathological features of cervical cancer was analyzed. Results：Mcl-lmRNA was higher in cervical cancer tissue when compared with corresponding normal tissue. Mcl-1 was overexpressed in cervical cancer tissue （positive rate of 53.3%） when compared with corresponding normal tissue （positive rate of 11.1%）. High expression of Mcl-1 was significantly correlated with histological grade,tumor size and lymph node involvement （P 〈 0.05）. Conclusion：Our results suggest that Mcl-1 may play an important role in cervical cancer and have potential as biomarker and therapeutic target.