含镧羟基磷灰石的细胞毒性研究

Cytotoxicity of lanthanum-substituted hydroxyapatite

目的 :体外研究含镧羟基磷灰石的细胞毒性。方法 :选用建系的L92 9成纤维细胞和原代培养的SD大鼠头盖骨细胞进行实验 ;采用中性红测试法、MTT测试法及碱性磷酸酶 (ALP)活性测试法研究含镧羟基磷灰石浸提液对细胞活性、增殖及功能表达的影响。结果 :中性红法、MTT法测试的活性细胞百分率 (RGR)均高于 75 % ;不同浓度材料浸提液与成骨细胞作用后ALP活性的A值 ,与阴性对照组相比较各组间无显著差异。结论

AIM: To evaluate the general cytotoxicity of lanthanum-substituted hydroxyapatites. METHODS: Two kinds of cells were used in this study, one was L929 fibroblast, the other was osteoblasts come from primary culture of rat calvaria. Neutral red test, MTT assay and Alkaline phosphatase (ALP) activity were carried out to establish cell viability, proliferation and differentiation. RESULTS: According to the neutral red test and MTT assay, the percentage of viable cells (RGR) was never lower than 75%; The OD value for ALP activity produced by osteoblast was similar for the negative control and the extracts. CONCLUSION: The lanthanum-substituted hydroxyapatites do not induce any cytotoxic effect.