米格列醇关键中间体6-(N-羟乙基)-6-脱氧-氨基山梨糖的生物合成工艺优化

Biosynthesis of key intermediate 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose of miglitol

目的:优化米格列醇关键中间体6-(N-羟乙基)-6-脱氧-氨基山梨糖的生物合成工艺。方法:以N-羟乙基葡糖胺作为原料,采用单因素试验方法优化工艺。分别考察了温度、转化时间、静息细胞浓度、底物浓度及起始pH对氧化葡萄糖酸杆菌静息细胞生物催化合成6-脱氧-6-羟乙基氨基山梨糖的影响。结果:在静息细胞浓度10%、底物浓度15%、起始pH 7.0,1000 mL摇瓶装液150 mL,30℃,200 r.min-1条件下振荡转化10 h,底物转化率可达93%。结论:该生物转化工艺切实可行。

Objective： To optimize the biosynthesis process of the key intermediate 6-（2-hydroxyethyl） a- mino-6-deoxy-L-sorbose of miglitol. Methods： Using N-（2-hydroxyethyl）-glucamine as starting material, single factor experiment design was applied to optimize the process. The effects of temperature, time, the concentration of resting cells, the concentration of substrate and the starting pH on biosynthesis of 6-（2-hydroxyethyl） amino-6-de- oxy-L-sorbose by Gluconobater oxydans＇s resting ceils were studied. Results： The transformation ratio could reach 93% when the concentration of resting cells and N-（2-hydroxyethyl）-glucamine were 10% and 15% respectively, the initial pH was 7.0 with 150 mL of medium being put into a flask of 1 000 mL, the temperature was 30℃ , and cultivation time was 10 h shaken at 200 r-min-1. Conclusion： This biotransformation process is practicable.