抑癌基因p16在胆管癌中表达的意义

Significance of tumor suppressor gene p16 expression in primary biliary cancer

目的研究 p16基因产物在人肝外胆管癌中的表达及其意义.方法应用免疫组织化学技术检测51例肝外胆管癌组织中p16表达,并分析其意义.结果 51例肝外胆管腺癌 P16蛋白的阳性率为43.1%,其阳性率在肿瘤的病理分级及神经侵润等方面差异有显著性(P<0.05),中位生存期在 P16蛋白阳性的肿瘤中比阴性的长(23 mo vs 8mo,P<0.05),P16蛋白阳性比 P16阴性肿瘤手术切除率高(52.9% vs 23.5%,P<0.05).结论 p16基因可能在胆管肿瘤的发生、发展中起重要作用,与胆管癌分化不良、神经侵润、生存时间及能否切除有密切关系.

AIM To examine the expression of p16 protein in primary biliary cancer and to determine the relationship between the expression of the gene product and biliary duct cancer. METHODS The expression of p16 protein was determined by immunohistechemical technique in 51 patients with biliary duct cancer. RESULTS Of the 51 patients examined,22 (43.1%) showed positive staining.The expression level of p16 protein remarkably correlated with tumor cell differentiation and nerve invasion of biliary duct cancer. Median survival time was shorter for those with p16 protein negative carcinomas than for those with p16 positive tumors (23 months vs 8 months P<0.05). Operative resection rate was higher for those with p16 protein positive carcinomas than for those with p16 protein negative tumors (56.3 % vs 22.1%,P<0.05). CONCLUSION p16 gene might play an important role in the occurrence and development of biliary duct cancer. The inactivation of p16 is remarkably correlated with the poorly-differentiated,nerve invasion of biliary cancer, survival time and optional operative procedures.