幽门螺杆菌感染T细胞亚群的表达及临床意义

Expression of T lymphocytes subsets in Helicobacter pylori causing infections and its clinical value

目的通过研究幽门螺杆菌(Hp)感染的慢性胃炎T淋巴细胞亚群(CD3+、CD4+、CD8+)的相对含量及CD4+/CD8+的比值,探讨其与慢性胃炎之间的关系与临床应用价值。方法利用流式细胞仪分别检测98例慢性胃炎患者和46例正常对照人群CD3+、CD4+、CD8+的相对含量,快速尿素酶法和14 C尿素呼气试验检测Hp。结果与正常对照组相比,慢性胃炎患者CD3+、CD4+、CD8+含量和CD4+/CD8+比值均呈显著性降低;与Hp阴性组相比,Hp阳性组仅CD3+的含量存在统计学降低;与轻度慢性胃炎组相比,CD3+的含量的中度组和重度组均存在显著性降低,重度组CD4+含量和CD4+/CD8+比值均显著性降低;与中度组相比较,轻度组仅CD3+的含量有显著升高,而重度组的CD3+、CD4+含量和CD4+/CD8+比值存在显著性降低。结论慢性胃炎患者存在一定的T淋巴细胞紊乱,其可能参与慢性胃炎的免疫应答过程。

OBJECTIVE To detect the T lymphocyte subsets,such as the relative percentage content of CD3＋,CD4＋,CD8＋ and the ratio of CD4＋/8＋ in Helicobacter pylori（Hp） infected chronic gastritis patients,so as to explore its correlation with the chronic gastritis and clinical value.METHODS The relative percentage content of CD3＋,CD4＋,CD8＋ in 98 chronic gastritis patients and 46 normal controls were detected by flow cytometry.The rapid urease and 14C urea breathe test were used to detect Hp.RESULTS Compared with the control group,the relative percentage content of CD3＋,CD4＋,CD8＋ and the ratio of CD4＋/8＋ in chronic gastritis patients was significantly decreased.Compared with Hp negative group,the relative percentage content of CD3＋ in Hp positive group was significantly decreased.Compared with mild chronic gastritis group,the relative percentage content of CD3＋ in the moderate group and severe group were significantly decreased,the relative percentage content of CD4＋ and the ratio of CD4＋/CD8＋ in severe group showed significantly decreased.Compared with the moderate group,the percentage content of CD3＋ in the mild group was significantly increased.The relative percentage of CD3＋,CD4＋ and the ratio of CD4＋/CD8＋ in the severe group were significantly decreased.CONCLUSION The patients with chronic gastritis show a certain disorder of T lymphocytes subsets,which may be associated with its involving in immune response process of chronic gastritis.