结直肠癌与人乳头状瘤病毒16型感染的相关性研究

Correlation between type 16 human papillomavirus infection and colorectal carcinoma

目的探讨结直肠癌与人乳头状瘤病毒(HPV)16型感染的相关性。方法入选2007年6月-2011年3月在医院住院治疗的结直肠癌患者75例,同时收集50名健康人群资料,采用免疫组化的方法检测病理组织中的人乳头状瘤病毒16型蛋白;根据手术病理结果,将所有患者分为低分化组21例、中分化组25例和高分化组29例;根据患者临床dukes分期分为A期27例、B期31例、C+D期17例,分别比较3组人乳头状瘤病毒16型感染情况。结果结直肠癌患者人乳头状瘤病毒16型感染阳性者51例,阳性率为68.0%;健康人群阳性例数6例,阳性率为12.0%,两组相比,差异有统计学意义(P<0.05);低分化组、中分化组和高分化组阳性率分别为66.7%、68.0%、65.5%,3组之间比较差异无统计学意义;A期、B期、C+D期3组的阳性率分别为55.6%、70.9%、82.3%,3组之间比较差异有统计学意义(P<0.05)。结论结直肠癌的发生和临床分期与人乳头状瘤病毒16型染密切相关,但是其恶性程度与人乳头状瘤病毒感染无显著相关。

OBJECTIVE To investigate the relationship between type 16 human papillomavirus infection and the development of colorectal carcinoma.METHODS A total of 75 colorectal carcinoma patients were enrolled in our hospital from Jul 2007 to Mar 2011,and 50 healthy persons were also enrolled.The colorectal tissues of both groups were analyzed by immunohistochemistry.All patients were divided into the poorly differentiated group（21 cases）,the oderately differentiated group（25 cases） and highly differentiated group（29 cases） according to their pathological findings.All patients were also divided into 27 cases in the dukes A stage,31 cases in the dukes stage B and 17 cases in the dukes C＋D stage according to dukes stages.The type 16 human papillomavirus infections of the three groups were compared.RESULTS There were 51 colorectal carcinoma patients who were infected with type 16 human papillomavirus with the positive rate of 68.0%;there were 6 positive healthy cases with the positive rate of 12.0%,the difference between the two groups was statistically significant（P0.05）,the positive rates of the poorly differentiated group,the moderately differentiated group and the highly differentiated group were 66.7%,68.0% and 65.5%,respectively,as compared with the three groups,the difference was not statistically significant;the positive rates of the stage A,stage B and stage C＋D were 55.6%,70.9%,and 82.3%,respectively,the difference among the three groups was statistically significant（P0.05）.CONCLUSION Type 16 human papillomavirus infection is closely related to the colorectal carcinoma as well its clinical stages,but its malignancy is not significantly correlated with human papillomavirus infection.