棓丙酯对细菌抗菌效应分析

Antibacterial effect of propylgallate

目的探讨棓丙酯对铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌和金黄色葡萄球菌的抗菌作用。方法采用微量肉汤稀释法和M-H平板转种法检测不同浓度的棓丙酯处理后几种细菌的生长情况,肉眼判断最低抑菌浓度(MIC)和最小杀菌浓度(MBC),并用酶标仪检测各细菌的吸光度值(OD值),用统计软件SPSS16.0对结果进行分析。结果棓丙酯对铜绿假单胞菌没有抑制作用,不同浓度的棓丙酯处理后细菌的OD值与阳性对照比较差异无统计学意义;棓丙酯能影响大肠埃希菌、肺炎克雷伯菌和金黄色葡萄球菌的生长,其中对大肠埃希菌的MIC为128～256μg/ml、MBC为256～512μg/ml;对肺炎克雷伯菌的MIC为512μg/ml、MBC为1024μg/ml;对金黄色葡萄球菌的MIC为256μg/ml、MBC为512μg/m;经统计分析低浓度棓丙酯处理后的大肠埃希菌、肺炎克雷伯菌和金黄色葡萄球菌OD值与阳性对照孔比较差异无统计学意义,高浓度的棓丙酯作用后细菌OD值明显减少,与阳性对照孔比较差异有统计学意义。结论棓丙酯能抑制大肠埃希菌、肺炎克雷伯菌和金黄色葡萄球菌的生长,可作为一种潜在抗菌药物用于临床上治疗这些细菌引起的感染。

OBJECTIVE To investigate the antibacterial effect of propylgallate on Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumoniae and Staphylococcus aureus.METHODS By the means of the broth dilution method and Mueller-Hinton agar dilution method,the growth of these bacteria after being treated by propylgallate with different concentrations was detected,the minimum inhibitory concentration（MIC） and minimum bactericidal concentration（MBC） were determined with naked eyes,the Microplate reader was used to detect the optical density（OD） of the cultured bacteria,the result was analyzed with SPSS software 16.0.RESULTS Propylgallate could not inhibit the growth of P.aeruginosa,there were no statistical difference between the optical density of the bacteria treated by propylgallate with different concentrations and the positive control;the propylgallate could influence the growth of E.coli,K.pneumoniae and S.aureus;the MIC of the propylgallate for E.coli varied from 128μg/ml to 256μg/ml,MBC from 256μg/ml to 512μg/ml;the MIC of the propylgallate for K.pneumoniae was 512μg/ml,MBC 1024μg/ml;the MIC of the propylgallate for S.aureus was 256μg/ml,MBC 512μg/ml;there was no statistical significance in the optical density of the bacteria treated by propylgallate with low concentration as compared with the positive control;the optical density decreased significantly after being treated with high concentration of propylgallate,with statistical significance（P0.05）.CONCLUSION Propylgallate can inhibit the growth of E.coli,K.pneumoniae and S.aureus,which can be used as a potential new drug for the clinical treatment of infections caused by these bacteria.