中药血散薯中总生物碱提取工艺优选

Optimization of Extraction Technology of Total Alkaloids from Stephania dielsiana

目的:优选血散薯总生物碱的提取工艺。方法:以总生物碱得率为指标,采用单因素和正交试验法考察乙醇体积分数、料液比、提取时间、pH对血散薯总生物碱得率的影响。结果:血散薯中总生物碱的最佳提取工艺为16倍量70%乙醇提取2.0 h,pH 2.0。结论:该优选工艺能较好的提取血散薯中的生物碱。

Objective：To optimize extraction technology of total alkaloids in Stephania dielsiana.Method： With yield of total alkaloids as index,effect of the concentration of ethanol,solid-liquid ratio,extraction time and pH on yield of total alkaloids was investigated by orthogonal test and single factor test.Result： The best extraction conditions were as follows：extracted 2 h with 16 times the amount of 70% ethanol,pH 2.0.Conclusion： This optimized technology was suitable for extraction of total alkaloids in S.dielsiana.